Fabrication of PLGA/HA (core)-collagen/amoxicillin (shell) nanofiber membranes through coaxial electrospinning for guided tissue regeneration
Fabrication of PLGA/HA (core)-collagen/amoxicillin (shell) nanofiber membranes through coaxial electrospinning for guided tissue regeneration
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DOI：
10.1016/j.compscitech.2016.02.005
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发表时间：
2016-03-23
影响因子：
9.1
通讯作者：
Tan, Quanchang
中科院分区：
文献类型：
--
作者：
Tang, Yufei;Chen, Lei;Tan, Quanchang
引导组织再生（GTR）膜是在骨缺损修复过程中用于在软组织和骨组织之间形成屏障的关键材料;然而，单一功能的屏障GTR膜不能满足临床要求。在本文中，我们提出了一种GTR膜的药物释放，骨引导，和屏障功能，这种膜是聚（乳酸-羟基乙酸）（PLGA）/羟基磷灰石（HA）（核心）胶原蛋白/阿莫西林（壳）共轴静电纺丝膜制造。每一个支架的外壳由胶原蛋白/阿莫西林组成，通过药物释放促进伤口愈合，其核心由PLGA/HA组成，阻断成纤维细胞生长到骨缺损中，促进骨生长。研究了壳层纺丝液中胶原含量对药物释放时间的影响，结果表明，当壳层纺丝液中胶原含量为4%时，阿莫西林的总释放时间可达40 h。降解8周后，在生物膜表面发生矿化，表明生物膜有诱导磷灰石沉积的能力。成纤维细胞也被共培养在核/壳双层膜的一侧，结果显示，培养侧的成纤维细胞生长良好;此外，在培养48 h后，在膜的另一侧没有观察到成纤维细胞。本工作开发的屏障膜可应用于牙科植入物和骨科移植的GTR。(C)2016爱思唯尔有限公司版权所有
Guided tissue regeneration (GTR) membranes are key materials used to create barriers between soft and bone tissues during bone defect repair; however, single-function barrier GTR membranes cannot meet clinical requirements. In this paper, we propose a GTR membrane with drug release, bone guidance, and barrier functions; this membrane is a poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) (core)collagen/amoxicillin (shell) nanofiber membrane fabricated by coaxial electrospinning. The shell of each nanofiber is composed of collagen/amoxicillin to promote wound healing through drug release, and its core is composed of PLGA/HA to block fibroblast growth into bone defects and promote bone growth. The effects of collagen content in the shell spinning solution on drug release time are studied, and results show that the total release time of amoxicillin can reach 40 h when a shell spinning solution containing 4 wt% collagen is used. Mineralization occurs on the nanofiber membrane surface after 8 weeks of degradation, indicating the ability of the membrane to induce apatite deposition. Fibroblasts were also co-cultured on one side of the core/shell nanofiber membranes, and results showed that fibroblasts on the cultured side grew well; moreover, no fibroblasts were observed on the opposite side of the membrane after 48 h of culture. The barrier membrane developed in this work may be applied in the GTR of dental implants and orthopedic transplants. (C) 2016 Elsevier Ltd. All rights reserved.