Morphogenesis of the lower incisor in the mouse from the bud to early bell stage.
Morphogenesis of the lower incisor in the mouse from the bud to early bell stage.
复制标题
小鼠下切牙从芽期到早钟期的形态发生。
DOI：
10.1387/ijdb.10610026
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
H. Lesot
中科院分区：
文献类型：
--
作者：
S. Kieffer;R. Peterkova;J. Vonesch;J. Ruch;M. Peterka;H. Lesot
利用计算机辅助三维重建技术从组织学切片研究小鼠下门牙的发育情况。在ED 13.0时，门牙仍处于萌芽阶段。在ED 13.5处，最初的帽被一个短的颈袢所分隔，其发育在唇侧进行，但在内侧大部分被阻滞。这种差异一直保持到ED 15.0。从ed16.0开始，达到了钟形阶段。中期在牙釉质器官和牙乳头中均有广泛的分布。在ED 13.5 ~ 14.0期间，唇颈环后方间质细胞凋亡逐渐增加，然后消失；这种细胞凋亡与切牙后生长无关。从ed13.5开始，在茎部观察到高的凋亡活性。在ED 13.5处，牙釉质器官的上皮-间质连接处观察到细胞凋亡的焦点区域，靠近门牙的未来尖端。在那里，内牙上皮向牙乳头形成球根状突起，使人联想到小鼠磨牙的继发牙釉质结。这种上皮突起仍保持在钟形期。与磨牙牙釉质结不同，切牙牙釉质结表现出特殊的特征：在ED 13.5时，切牙上皮细胞的同心排列不那么突出，细胞凋亡的发生非常短暂。低凋亡活性牙釉质结的消失和牙突的维持提示了啮齿动物切牙特有的组织重组。
The development of the lower incisor in the mouse was investigated from histological sections using computer-aided 3D reconstructions. At ED 13.0, the incisor was still at the bud stage. At ED 13.5, the initial cap was delimited by a short cervical loop, the development of which proceeded on the labial side, but was largely retarded on the medial side. This difference was maintained up to ED 15.0. From ED 16.0, the bell stage was achieved. Metaphases had a ubiquitous distribution both in the enamel organ and in the dental papilla from the bud to early bell stage. Apoptosis gradually increased in the mesenchyme posteriorly to the labial cervical loop from ED 13.5 to 14.0 and then disappeared; this apoptosis was not related to the posterior growth of the incisor. From ED 13.5, a high apoptotic activity was observed in the stalk. A focal area of apoptosis was observed at ED 13.5 in the enamel organ, approaching the epithelio-mesenchymal junction at the future tip of the incisor. There, the inner dental epithelium formed a bulbous protrusion towards dental papilla, reminiscent of the secondary enamel knot of mouse molars. This epithelial protrusion was still maintained at the bell stage. The enamel knot in the incisor demonstrated specific features, different from those characterizing the enamel knot in the molar: the concentric arrangement of epithelial cells was much less prominent and the occurrence of apoptosis was very transitory in the incisor at ED 13.5. The disappearance of the enamel knot despite a low apoptotic activity and the maintenance of the protrusion suggested a histological reorganization specific for rodent incisor.
DOI：
10.1387/ijdb.7848830
发表时间：
1994-09
期刊：
The International journal of developmental biology
影响因子：
--
作者：
J. Jernvall;P. Kettunen;I. Karavanova;L. Martin;Irma Thesleff
通讯作者：
J. Jernvall;P. Kettunen;I. Karavanova;L. Martin;Irma Thesleff