Compartmentalised inducible nitric-oxide synthase activity in septic shock
Compartmentalised inducible nitric-oxide synthase activity in septic shock
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DOI：
10.1016/s0140-6736(00)02063-8
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发表时间：
2000-04-01
期刊：
影响因子：
168.9
通讯作者：
Bellissant, E
中科院分区：
文献类型：
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作者：
Annane, D;Sanquer, S;Bellissant, E
背景先前的实验研究支持诱导型一氧化氮合酶(INOS)在严重脓毒症发病机制中的作用。目的探讨感染性休克患者不同组织中诱导型一氧化氮合酶(INOS)活性的变化。从正常、炎症和坏死区获取皮肤、肌肉、脂肪和动脉样本，以测定iNOS活性，以及肿瘤坏死因子α(TNFα)和白介素1β(IL-1β)的浓度，在两名患者中，还检测了与引起脓毒症的微生物孵育的外周血单个核细胞(PRMC)或与IL-1β孵育的化脓性腹腔液中分离的巨噬细胞的iNOS活性。结果：肌肉坏死区的iNOS活性比正常区和炎症区高71倍[95%CI 20-259]比正常区高69倍[19-246]比炎症区高；
Background Previous experimental studies support a role for inducible nitric-oxide synthase (iNOS) in the pathogenesis of severe sepsis. The aim of the study was to characterise iNOS activity in different tissues in patients with septic shock.Methods 13 consecutive patients with septic shock caused by cellulitis were enrolled. Skin, muscle, fat, and artery samples were obtained from normal, inflamed, and putrescent areas to measure iNOS activity, and concentrations of tumour necrosis factor alpha (TNF alpha) and interleukin 1 beta (IL-1 beta), In two patients, iNOS activity was also assessed in peripheral blood mononuclear cells (PRMC) incubated with microorganisms causing the sepsis, or in macrophages isolated from suppurating peritoneal fluid incubated with IL-1 beta.Findings: Compared with normal and inflamed areas, iNOS activity was increased in putrescent areas for muscle (71-fold [95% CI 20-259] vs normal areas, 69-fold [19-246] vs inflamed areas; p