Lysis of human red blood cells .4. Comparison of in vitro and in vivo hemolysis data
Lysis of human red blood cells .4. Comparison of in vitro and in vivo hemolysis data
复制标题
DOI：
10.1021/js970184o
复制
发表时间：
1997-11-01
影响因子：
3.8
通讯作者：
Yalkowsky, SH
中科院分区：
文献类型：
--
作者：
Krzyzaniak, JF;Núñez, FAA;Yalkowsky, SH
作者开发的动态体外方法以及其他可用的体外方法被用于确定几种之前已在体内进行评估的助溶剂载体所诱导的溶血程度。针对每种载体所生成的体外数据与体内溶血数据进行了比较，以评估该方法预估体内溶血的能力。结果表明，动态方法所生成的体外数据与每种载体的体内数据一致。因此，注射后制剂诱导血管内溶血的可能性可通过这种动态体外方法确定。有了这些信息，就能够更容易地制备溶血安全的制剂。
The dynamic in vitro method developed by the authors and other available in vitro methods were used to determine the degree of hemolysis induced by several cosolvent vehicles that have previously been evaluated in vivo. The in vitro data generated for each of these vehicles was compared with the in vivo hemolysis data to assess the ability of the method to estimate in vivo hemolysis. The results show that the in vitro data generated by the dynamic method are in agreement with the in vivo data for each vehicle. Therefore, the potential for formulations to induce intravascular hemolysis after injection can be determined by this dynamic in vitro method. With this information, hemolytically safe formulations can more easily be prepared.