Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
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发育中的禽类结直肠中肠神经嵴细胞的免疫表型特征。
DOI：
10.1002/dvdy.23767
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Goldstein,AllanM
中科院分区：
文献类型：
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作者：
Nagy,Nandor;Burns,AlanJ;Goldstein,AllanM
背景：肠神经系统（ENS）由神经嵴来源的细胞发育而来，这些细胞沿着肠迁移，形成神经元和神经胶质两个神经丛。虽然ENS发育的主要特征在物种间是保守的，但存在微小差异，特别是在结直肠中。鉴于胚胎学和疾病相关的重要性，远端ENS，本研究的目的是表征的迁移和分化的肠神经嵴衍生细胞（ENCC）在结肠直肠的禽embryos.Results：使用正常鸡胚和迷走神经管移植从绿色荧光蛋白（GFP）转基因鸡胚，我们发现ENCC进入结肠在胚胎天（E）6.5，定植完成E8。未分化的ENCC在波前表达HNK-1、N-钙粘蛋白、Sox 10、p75和L1 CAM。到E7，分化开始于近端结肠，L1 CAM和Sox 10分别限于神经元和神经胶质谱系。通过E8，多个分化标志物沿沿着整个colorectal.Conclusions表达：我们的研究结果建立了模式的ENCC迁移和分化在鸡结肠直肠，证明跨物种的标志物表达的保护，突出了一系列的标志物，包括神经元细胞粘附分子，标记细胞在波前，并提供了一个框架，为今后的研究在禽ENS发展。Developmental Dynamics 241：842-851，2012.© 2012 Wiley Periodicals，Inc.
Background:The enteric nervous system (ENS) develops from neural crest‐derived cells that migrate along the intestine to form two plexuses of neurons and glia. While the major features of ENS development are conserved across species, minor differences exist, especially in the colorectum. Given the embryologic and disease‐related importance of the distal ENS, the aim of this study was to characterize the migration and differentiation of enteric neural crest‐derived cells (ENCCs) in the colorectum of avian embryos.Results:Using normal chick embryos and vagal neural tube transplants from green fluorescent protein (GFP) ‐transgenic chick embryos, we find ENCCs entering the colon at embryonic day (E) 6.5, with colonization complete by E8. Undifferentiated ENCCs at the wavefront express HNK‐1, N‐cadherin, Sox10, p75, and L1CAM. By E7, differentiation begins in the proximal colon, with L1CAM and Sox10 becoming restricted to neuronal and glial lineages, respectively. By E8, multiple markers of differentiation are expressed along the entire colorectum.Conclusions:Our results establish the pattern of ENCC migration and differentiation in the chick colorectum, demonstrate the conservation of marker expression across species, highlight a range of markers, including neuronal cell adhesion molecules, which label cells at the wavefront, and provide a framework for future studies in avian ENS development. Developmental Dynamics 241:842–851, 2012. © 2012 Wiley Periodicals, Inc.