Apoptosis and its correlation with proliferative activity in rectal cancer
Apoptosis and its correlation with proliferative activity in rectal cancer
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DOI：
10.1002/jso.10063
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发表时间：
2002-04-01
影响因子：
2.5
通讯作者：
Choi, KW
中科院分区：
文献类型：
--
作者：
Kim, YH;Lee, JH;Choi, KW
背景和目的：细胞凋亡和细胞增殖正常调控的改变是多步结直肠癌发生的重要因素。本研究旨在探讨直肠癌组织中细胞凋亡和细胞增殖的频率及其与临床病理参数和bcl2、p53表达的关系。方法：对57例直肠癌石蜡包埋组织进行末端脱氧核苷酸转移酶(TdT)介导的dUTP缺口末端标记(TUNEL)染色和Ki-67、bcl2、p53免疫组织化学染色。结果：细胞凋亡指数(AI)与增殖指数(PI)呈正相关(P=0.276，P=0.038)。有淋巴结转移的直肠癌组织中细胞凋亡和细胞增殖均较多见(P=0.045和0.010)。然而，AI/PI的比率并不因节点状态而异。Dukes分期与AI、PI之间无相关性。直肠癌组织中细胞凋亡率与bcl2表达呈负相关，与P53状态无关。细胞增殖与bcl2、p53的表达无相关性。结论：直肠癌对细胞凋亡的易感性与肿瘤细胞的增殖活性及高保真转换率密切相关，可能与直肠癌的淋巴转移有关。
Background and Objectives: Alterations in the normal control of apoptosis and cell proliferation are important factors in multistep colorectal carcinogenesis. The aim of this study was to determine the frequency of apoptosis and cell proliferation in rectal cancers and to examine their relationship to clinicopathological variables and expression of bcl-2 and p53.Methods: Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining and immunohistochemical staining for Ki-67, bcl-2, and p53 were performed on paraffin-embedded tissue samples of 57 rectal cancers.Results: There was a positive linear correlation between apoptotic index (AI) and proliferative index (PI) (gamma=0.276, P=0.038). Both apoptosis and cell proliferation were more frequently found in rectal cancers with lymph node metastasis (P=0.045 and 0.010, respectively). However, the ratio of AI and PI was not different by nodal status. There was no association between Dukes stage and AI or PI. The frequency of apoptosis was inversely related to the expression of bcl-2, but was not related to the p53 status of rectal cancer. There were no association between cell proliferation and the expression of bcl-2 or p53.Conclusions: Our results suggest that the susceptibility to apoptosis in rectal cancer is clearly related to the proliferative activity and hi-fi turnover rate of tumor cells may contribute to lymph node metastasis.