Distinct pathways for modification of the bacterial cell wall by non-canonical D-amino acids
Distinct pathways for modification of the bacterial cell wall by non-canonical D-amino acids
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DOI：
10.1038/emboj.2011.246
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发表时间：
2011-08-17
期刊：
影响因子：
11.4
通讯作者：
Waldor, Matthew K.
中科院分区：
文献类型：
--
作者：
Cava, Felipe;de Pedro, Miguel A.;Waldor, Matthew K.
在稳定期产生非典型D-氨基酸（NCDAA）促进肽聚糖（PG）的重塑，所述肽聚糖是构成细菌细胞壁的聚合物。NCDAA生产的损害导致PG的过度积累和对渗透压休克的超敏反应;然而，这些表型的机制基础以前没有确定。在这里，我们表明，将NCDAA纳入PG是一个关键的手段，NCDAA控制PG的丰度和强度。我们在体外鉴定并重建了两个（至少三个）介导NCDAA掺入的不同过程。不同的细菌门通过成熟PG的周质编辑或通过掺入胞质溶胶中的PG前体亚基将NCDAA掺入其细胞壁中。霍乱弧菌中NCDAA的产生需要应激反应因子RpoS，这表明NCDAA可能有助于细菌应对各种环境挑战。不同的细菌，包括非生产者，将NCDAA的广泛能力表明，这些氨基酸可能作为自分泌和旁分泌样调节剂的化学和物理性质的细胞壁在微生物群落。The EMBO Journal（2011）30，3442-3453. doi：10.1038/doj.2011.246; 2011年7月26日在线发布
Production of non-canonical D-amino acids (NCDAAs) in stationary phase promotes remodelling of peptidoglycan (PG), the polymer that comprises the bacterial cell wall. Impairment of NCDAAs production leads to excessive accumulation of PG and hypersensitivity to osmotic shock; however, the mechanistic bases for these phenotypes were not previously determined. Here, we show that incorporation of NCDAAs into PG is a critical means by which NCDAAs control PG abundance and strength. We identified and reconstituted in vitro two (of at least three) distinct processes that mediate NCDAA incorporation. Diverse bacterial phyla incorporate NCDAAs into their cell walls, either through periplasmic editing of the mature PG or via incorporation into PG precursor subunits in the cytosol. Production of NCDAAs in Vibrio cholerae requires the stress response sigma factor RpoS, suggesting that NCDAAs may aid bacteria in responding to varied environmental challenges. The widespread capacity of diverse bacteria, including non-producers, to incorporate NCDAAs suggests that these amino acids may serve as both autocrine-and paracrine-like regulators of chemical and physical properties of the cell wall in microbial communities. The EMBO Journal (2011) 30, 3442-3453. doi:10.1038/emboj.2011.246; Published online 26 July 2011