Expression Profiling of Chloroplast-encoded Proteins in Rice Leaves at Different Growth Stages
Expression Profiling of Chloroplast-encoded Proteins in Rice Leaves at Different Growth Stages
复制标题
水稻不同生长阶段叶绿体编码蛋白的表达谱
DOI：
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发表时间：
2011
影响因子：
0.3
通讯作者：
Liu Guo-Zhen
中科院分区：
文献类型：
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作者：
Chen Hao;Liu Sheng-Nan;Wu Lin;Liu Guo-Zhen
叶绿体是植物将光能转化为化学能的重要细胞器，目前许多植物的叶绿体全基因组序列已被公布，对叶绿体中的生物学过程的认识也已积累，然而，关于叶绿体编码蛋白质表达的信息有限，从叶绿体中选择了15个叶绿体基因，结果表明，7种光合相关蛋白在水稻叶片中的表达模式基本一致，且随着叶片的生长，其表达量逐渐增加，到孕穗期和开花期，其表达量逐渐增加，到开花期，其表达量逐渐减少，而到孕穗期，其表达量逐渐减少。光合作用相关蛋白的表达量与光合作用之间存在一定的相关性，与DNA复制相关的4种RNA聚合酶在苗期的表达量最高，说明它们在苗期就发挥了作用，而与DNA复制相关的4种RNA聚合酶在苗期的表达量最高，4 NADH脱氢酶有两种表达模式，亚基-2和亚基-4在苗期叶长为1cm时表达量最高，亚基-4在苗期叶长为1cm时表达量最高，亚基-2和亚基-4在苗期叶长为1cm时表达量最高，亚基-4在苗期叶长为1cm时表达量最高。亚基-5和-7在苗期中后期达到各自的水平，说明它们在苗期的功能不同，综合来看，本实验以直接、相对定量的方式揭示了水稻叶绿体编码蛋白在水稻不同生育期叶片中的表达模式及其与生长过程的相关性，为进一步研究其功能提供了重要信息。
Chloroplast is the critical organelle for transforming light energy into chemical energy in plant.At present the whole genome sequences of chloroplast of many plants have been released and the knowledge about biological processes in chloroplast has been accumulated.However,limited information regarding the expression of chloroplast-encoded proteins is available.Fifteen chloroplast genes were selected from chloroplast-encoded proteins and the expression profiling in different stages of rice leaves were investigated by Western blotting.The results indicated that the expression patterns of 7 photosynthesis-related proteins are similar and the expression level increase steadily as leaves grow taller.Until the booting and flowering stages,the expression reached the highest-level,and then decreased at filling stage.The correlation between the expression of photosynthesis-related proteins and the photosynthesis was observed.The expression levels of 4 RNA polymerase related to DNA replication were at their highest-level at seedling stage,which indicated that they perform their functions during the early stage.However,4 NADH dehydrogenase exhibited two kinds of expression patterns.Subunit-2 and-4 reached their highest-level when the leaves were 1cm in length during seedling stage.While,subunit-5 and-7 reached theirs in middle and later stage of seedling.The results implied their different functions during seedling stages.Taking together,this experiment revealed the expression patterns of rice chloroplast-encoded proteins in rice leaves at different growth stages and the correlation between the proteins and the growing process in a direct and relative quantitative way,and provided significant information for further research of their function.