The putative serine protease inhibitor (serpin) genes encoded on Echinococcus multilocularis genome and their expressions in metacestodal stage.
The putative serine protease inhibitor (serpin) genes encoded on Echinococcus multilocularis genome and their expressions in metacestodal stage.
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DOI：
10.1016/j.vetpar.2016.11.019
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发表时间：
2017-01
影响因子：
2.6
通讯作者：
M. Sasaki;Y. Sako
中科院分区：
文献类型：
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作者：
M. Sasaki;Y. Sako
在多房棘球绦虫中，除了已被鉴定的丝氨酸蛋白酶抑制剂Emu基因外，还鉴定出两个推定的丝氨酸蛋白酶抑制剂基因，分别命名为丝氨酸蛋白酶抑制剂2Emu和丝氨酸蛋白酶抑制剂3Emu。用多克隆抗体对serpinEmu、推定的serpin2Emu蛋白和推定的serpin3Emu蛋白进行Western印迹分析表明，这三种蛋白均定位于E.多室后绦虫此外，寄生虫组织的免疫染色表明，所有三种蛋白质都定位在germinal层。
Two putative serpin genes were identified inEchinococcus multilocularis, in addition to the already reportedserpinEmu, and were designated asserpin2Emuandserpin3Emu. Western blot analysis using polyclonal antibodies against serpinEmu, putative serpin2Emu protein, and putative serpin3Emu protein indicated that all three proteins were localized in both intracellular and excretory-secretory (ES) fractions ofE. multilocularismetacestodes. In addition, immune staining of parasite tissue indicated that all three proteins were localized at the germinal layer.