Mechanical Signals Induce Dedifferentiation of Mature Adipocytes and Increase the Retention Rate of Fat Grafts
Mechanical Signals Induce Dedifferentiation of Mature Adipocytes and Increase the Retention Rate of Fat Grafts
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机械信号诱导成熟脂肪细胞去分化并提高脂肪移植物的保留率
DOI：
10.1097/prs.0000000000006272
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发表时间：
2019
影响因子：
3.6
通讯作者：
Liao Yunjun
中科院分区：
文献类型：
--
作者：
Ma Jingjing;Xia Jing;Gao Jianhua;Lu Feng;Liao Yunjun
背景：成熟脂肪细胞在体内应用软组织扩张器后去分化。去分化的脂肪细胞可以增殖和再分化。本研究采用组织扩张器对脂肪瓣进行预处理，以提高脂肪移植后的留存率。方法：将软组织扩张器和硅胶片分别植入大鼠左、右腹股沟脂肪垫下方。扩张7天后，将来自垫的脂肪组织移植到背部皮肤下。在不同的时间点收集样本，并进行组织学、免疫组织化学和基因表达分析。在520 Pa压力下体外培养成熟脂肪细胞。细胞形态，细胞骨架，和机械信号相关蛋白的表达的变化进行了investigated.Results：在脂肪瓣的压力增加到25 kPa的扩张。成熟脂肪细胞在扩增后去分化。移植后1周，扩张组血管内皮生长因子表达高于扩张组（P< 0.05）。移植后12周，扩张组的留存率（56± 3%）高于对照组（32± 3%）（p< 0.05），存活/再生区（p< 0.01）更宽。成熟脂肪细胞的脂质含量逐渐下降，在增加的压力下培养，这些细胞恢复增殖能力。结论：机械信号可诱导成熟脂肪细胞的去分化。去分化脂肪细胞作为种子细胞增加脂肪移植物的留存率。
Background:Mature adipocytes dedifferentiate in vivo on application of a soft-tissue expander. Dedifferentiated adipocytes can proliferate and redifferentiate. This study used tissue expanders to pretreat adipose flaps, to increase the retention rate after fat graft.Methods:A soft-tissue expander and silicone sheet were implanted beneath the left and right inguinal fat pads of rats, respectively. After 7 days of expansion, the adipose tissue derived from the pads was transplanted beneath dorsal skin. Samples were harvested at various time points, and histologic, immunohistochemical, and gene expression analyses were conducted. Mature adipocytes were cultured in vitro under a pressure of 520 Pa. Changes in cell morphology, the cytoskeleton, and expression of mechanical signal–related proteins were investigated.Results:Pressure in adipose flaps increased to 25 kPa on expansion. Mature adipocytes dedifferentiated following expansion. At 1 week after transplantation, the expression of vascular endothelial growth factor (p< 0.05) was higher in the expanded group. The retention rate at 12 weeks after transplantation was higher in the expanded group (56±3 percent) than in the control group (32±3 percent)(p< 0.05), and the surviving/regenerating zones (p< 0.01) were wider. The lipid content of mature adipocytes gradually decreased on culture under increased pressure, and these cells regained a proliferative capacity. This was accompanied by increased expression of mechanical signal--related proteins (p< 0.05).Conclusions:Mechanical signals may induce dedifferentiation of mature adipocytes. Dedifferentiated adipocytes increase the retention rate of fat grafts by acting as seed cells.