Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells.
Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells.
复制标题
聚丙烯酸调控合成棒状碳酸钙纳米颗粒诱导 MC3T3-E1 细胞成骨分化
DOI：
10.3390/ijms17050639
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发表时间：
2016-05-06
影响因子：
5.6
通讯作者：
Kong X
中科院分区：
文献类型：
--
作者：
Yang W;Yao C;Cui Z;Luo D;Lee IS;Yao J;Chen C;Kong X
碳酸钙，特别是纳米碳酸钙，由于其良好的生物降解性和骨传导性，被认为是一种很好的骨再生材料。在本研究中，通过聚丙烯酸的调节，获得了具有所需水分散性的棒状碳酸钙纳米颗粒（Rod-CC NPs）。表征结果显示，棒状-CC纳米颗粒的平均长度为240 nm，宽度为90 nm，平均纵横比为2.60，负电位为-22.25 ± 0.35 mV。降解研究表明，在磷酸盐缓冲盐水（PBS）溶液中，纳米颗粒在pH 7.4下降解23%，在pH 5.6下降解45%。当与MC 3 T3-E1细胞一起培养时，Rod-CC NPs表现出对成骨细胞增殖的积极作用。碱性磷酸酶（ALP）活性测定以及骨钙素（OCN）和骨唾液蛋白（BSP）表达的观察表明纳米颗粒可以诱导MC 3 T3-E1细胞分化。我们的研究开发了分散良好的棒状碳酸钙纳米粒子，具有很大的潜力，用于骨再生。
Calcium carbonate, especially with nanostructure, has been considered as a good candidate material for bone regeneration due to its excellent biodegradability and osteoconductivity. In this study, rod-like calcium carbonate nanoparticles (Rod-CC NPs) with desired water dispersibility were achieved with the regulation of poly (acrylic acid). Characterization results revealed that the Rod-CC NPs had an average length of 240 nm, a width of 90 nm with an average aspect ratio of 2.60 and a negative ζ-potential of −22.25 ± 0.35 mV. The degradation study illustrated the nanoparticles degraded 23% at pH 7.4 and 45% at pH 5.6 in phosphate-buffered saline (PBS) solution within three months. When cultured with MC3T3-E1 cells, the Rod-CC NPs exhibited a positive effect on the proliferation of osteoblast cells. Alkaline phosphatase (ALP) activity assays together with the osteocalcin (OCN) and bone sialoprotein (BSP) expression observations demonstrated the nanoparticles could induce the differentiation of MC3T3-E1 cells. Our study developed well-dispersed rod-like calcium carbonate nanoparticles which have great potential to be used in bone regeneration.