Monitoring tetracycline through a solid-state nanopore sensor.
Monitoring tetracycline through a solid-state nanopore sensor.
复制标题
通过固态纳米孔传感器监测四环素
DOI：
10.1038/srep27959
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发表时间：
2016-06-16
影响因子：
4.6
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Zhang Y;Chen Y;Fu Y;Ying C;Feng Y;Huang Q;Wang C;Pei DS;Wang D
抗生素作为一种新兴的环境污染物，广泛应用于人用和兽用药物中。本文报道了一种基于Tet-off和Tet-on体系的四环素固体纳米孔传感检测方法。在四环素的作用下，rtTA（reversetetracycline-controlledtrans-activator）和TRE（TetracyclineResponsiveElement）可相互结合形成复合物。当配合物通过直径为8 ~ 9 nm的纳米孔时，可检测到浓度为2 ~ 2000 ng/mL的泰特。根据Logistic模型，四环素对rtTA和TRE的生长可分为三个生长区。缓慢生长区为0-39.5 ng/mL。快速生长区为39.5 - 529.7 ng/mL。饱和区> 529.7 ng/mL。与以前的方法相比，纳米孔传感器可以在单分子水平上检测和定量这些不同种类的分子。
Antibiotics as emerging environmental contaminants, are widely used in both human and veterinary medicines. A solid-state nanopore sensing method is reported in this article to detect Tetracycline, which is based on Tet-off and Tet-on systems. rtTA (reverse tetracycline-controlled trans-activator) and TRE (Tetracycline Responsive Element) could bind each other under the action of Tetracycline to form one complex. When the complex passes through nanopores with 8 ~ 9 nanometers in diameter, we could detect the concentrations of Tet from 2 ng/mL to 2000 ng/mL. According to the Logistic model, we could define three growth zones of Tetracycline for rtTA and TRE. The slow growth zone is 0–39.5 ng/mL. The rapid growth zone is 39.5−529.7 ng/mL. The saturated zone is > 529.7 ng/mL. Compared to the previous methods, the nanopore sensor could detect and quantify these different kinds of molecule at the single-molecule level.