Effects of direct-fed microbials on growth performance, gut morphometry, and immune characteristics in broiler chickens
Effects of direct-fed microbials on growth performance, gut morphometry, and immune characteristics in broiler chickens
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DOI：
10.3382/ps.2009-00418
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发表时间：
2010-02-01
期刊：
影响因子：
4.4
通讯作者：
Siragusa, G. R.
中科院分区：
文献类型：
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作者：
Lee, K. W.;Lee, S. H.;Siragusa, G. R.
本研究旨在比较连续22天添加芽孢杆菌的肉鸡的生长性能、肠道形态计量学以及局部和全身免疫参数。作为直接饲喂微生物（DFM）、掺入3DFM的商业产品或非补充饮食。直接喂养微生物没有显着改变BW增益，大多数未能影响血清抗体水平的免疫重组艾美耳球虫蛋白。然而，改变肠道形态测量是显而易见的，在DFM喂养的鸡揭示了增加绒毛高度和隐窝深度相比，非DFM喂养的控制。此外，血清中的α-1-酸性糖蛋白作为炎症标志物的DFM喂养的鸟类减少，而脾淋巴细胞增殖，肠上皮内淋巴细胞亚群，上皮内淋巴细胞中的细胞因子mRNA水平增加，减少，或与对照组相比，取决于DFM使用不变。这些结果为进一步研究DFM作为免疫调节剂提高肉鸡对肠道病原菌的保护性免疫提供了科学依据。
This study was conducted to compare growth performance, gut morphometry, and parameters of local and systemic immunity in broiler chickens fed for 22 consecutive days with a diet supplemented with Bacillus spp. as direct-fed microbials (DFM), a commercial product incorporating 3 DFM, or a non-supplemented diet. Direct-fed microbials did not significantly modify BW gain and most failed to affect serum antibody levels in response to immunization with a recombinant Eimeria protein. However, altered intestinal morphometric measurements were readily apparent in DFM-fed chickens as revealed by increased villus height and crypt depth compared with non-DFM-fed controls. In addition, serum levels of alpha-1-acid glycoprotein as an inflammatory marker were reduced in DFM-fed birds, whereas splenic lymphocyte proliferation, intestine intraepithelial lymphocyte subpopulations, and cytokine mRNA levels in intraepithelial lymphocytes were increased, decreased, or unchanged compared with controls depending on the DFM used. These results provide a rational scientific basis for future studies to investigate DFM as immunomodulating agents to enhance host protective immunity against enteric pathogens in broiler chickens.