Evaluation of the safety, immunogenicity and efficacy of three capripoxvirus vaccine strains against lumpy skin disease virus
Evaluation of the safety, immunogenicity and efficacy of three capripoxvirus vaccine strains against lumpy skin disease virus
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DOI：
10.1016/j.vaccine.2015.01.035
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发表时间：
2015-06-22
期刊：
影响因子：
5.5
通讯作者：
Tuppurainen, Eeva S. M.
中科院分区：
文献类型：
--
作者：
Gari, Getachew;Abie, Getnet;Tuppurainen, Eeva S. M.
结合疫苗激发实验和现场接种动物免疫反应监测，评估了三种针对牛块状皮肤病 (LSD) 的市售疫苗的安全性、免疫原性和有效性。研究中评估的三种疫苗包括两种当地生产的（埃塞俄比亚）疫苗（块状皮肤病病毒（LSDV）Neethling和肯尼亚绵羊和山羊痘（KSGP）O-180株疫苗）和约旦生物工业中心（JOVAC）生产的戈尔根山羊痘（GTP）疫苗。后一种疫苗首次在牛身上针对 LSDV 进行了评估。埃塞俄比亚 Neethling 和 KSGPO-180 疫苗未能为牛提供针对 LSDV 的保护，而 Gorgan GTP 疫苗则可以保护所有接种疫苗的小牛免受 LSD 临床症状的影响。两种剂量水平之间检测到的保护功效没有显着差异（KSGP、Neethling 和 Gorgan 疫苗分别为 P=0.2、P=0.25 和 P=0.1）。此外，Gorgan GTP 疫苗接种的牛表现出更强的细胞免疫反应水平，使用疫苗接种部位的迟发型超敏反应 (DTH) 反应进行测量，表明与其他两种疫苗相比，GTPV 疫苗在牛体内产生的免疫原性水平更高。这项研究首次表明，Gorgan GTP 疫苗可以有效保护牛免受 LSDV 感染，而 Neethling 和 KSGP O-180 疫苗则没有保护作用。结果强调需要对埃塞俄比亚用于疫苗生产的 Neethling 和 KSGP O-180 疫苗种子病毒进行分子表征。此外，埃塞俄比亚 LSD Neethling 和 KSGP O-180 疫苗的效力和功效测试过程应重新评估。 (C) 2015 Elsevier Ltd. 保留所有权利。
The safety, immunogenicity and efficacy of three commercially available vaccines against lumpy skin disease (LSD) in cattle have been evaluated using a combination of vaccine challenge experiments and the monitoring of immune responses in vaccinated animals in the field. The three vaccines evaluated in the study included two locally produced (Ethiopian) vaccines (lumpy skin disease virus (LSDV) Neethling and Kenyan sheep and goat pox (KSGP) O-180 strain vaccines) and a Gorgan goat pox (GTP) vaccine manufactured by Jordan Bio-Industries Centre (JOVAC). The latter vaccine was evaluated for the first time in cattle against LSDV. The Ethiopian Neethling and KSGPO-180 vaccines failed to provide protection in cattle against LSDV, whereas the Gorgan GTP vaccine protected all the vaccinated calves from clinical signs of LSD. There was no significant difference in protective efficacy detected between two dosage levels (P=0.2, P=0.25, and P=0.1 for KSGP, Neethling and Gorgan vaccines, respectively). Additionally, the Gorgan GTP vaccinated cattle showed stronger levels of cellular immune responses measured using Delayed-Type Hypersensitivity (DTH) reactions at the vaccination site indicating higher levels of immunogenicity produced by the GTPV vaccine in cattle, as opposed to the other two vaccines. This study indicated, for the first time, that the Gorgan GTP vaccine can effectively protect cattle against LSDV and that the Neethling and KSGP O-180 vaccine were not protective. The results emphasise the need for molecular characterization of the Neethling and KSGP O-180 vaccine seed viruses used for vaccine production in Ethiopia. In addition, the potency and efficacy testing process of the Ethiopian LSD Neethling and KSGP O-180 vaccines should be re-evaluated. (C) 2015 Elsevier Ltd. All rights reserved.