Effectiveness of the DNA barcoding approach for closely related conifers discrimination: A case study of the Pinus mugo complex
Effectiveness of the DNA barcoding approach for closely related conifers discrimination: A case study of the Pinus mugo complex
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DOI：
10.1016/j.crvi.2017.06.002
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发表时间：
2017-06-01
影响因子：
2
通讯作者：
Chudzinska, Ewa
中科院分区：
文献类型：
--
作者：
Celinski, Konrad;Kijak, Hanna;Chudzinska, Ewa
DNA条形码是一种标准而有效的方法，经常用于鉴定、区分和发现新物种。虽然这种方法是非常有用的分类世界的生物多样性，很少有人知道它的有用性条形码在较低的分类水平和它的歧视率密切相关的物种，如针叶树。在这项研究中，我们比较了8个叶绿体DNA条形码区域（matK，rbcL，trnH-psbA，trnL-trnF，rp 120-rps 18，trnV，ycf 1，ycf 2）的遗传变异在17种针叶树-三个密切相关的松从松mugo复合体和14个更远的针叶树代表两个属和松科的四个部分。在本研究中分析的单个和多个DNA条形码区域的区分率使用树构建和PWG距离方法来估计。DNA条形码方法的有用性进行了评估和讨论，用于分析和解决分类学上的不一致性密切相关的和更远的遗传学上的针叶树。(C)2017年，科学院。由Elsevier Masson SAS出版。All rights reserved.
DNA barcoding is a standard and efficient method, frequently used for identification, discrimination and discovery of new species. Although this approach is very useful for classifying the world's biodiversity, little is known about its usefulness in barcoding at lower taxonomic level and its discrimination rate for closely related species, like conifers. In this study, we compared the genetic variation of eight chloroplast DNA barcode regions (matK, rbcL, trnH-psbA, trnL-trnF, rpl20-rps18, trnV, ycf1, ycf2) in 17 conifers - three closely related pines from Pinus mugo complex and 14 more distant conifers representing two genera and four sections of the Pinaceae family. The discrimination rate for a single and for multiple DNA barcode regions analyzed in this study was estimated using the Tree-Building and PWG-Distance methods. The usefulness of the DNA barcoding approach for analyzing and resolving taxonomic inconsistency among closely related and more phylogenetically distant conifers was evaluated and discussed. (C) 2017 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.