Immunochemical characterization and ultrastructural localization of chondroitin sulfates and keratan sulfate in embryonic chick bone marrow.
Immunochemical characterization and ultrastructural localization of chondroitin sulfates and keratan sulfate in embryonic chick bone marrow.
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胚胎鸡骨髓中硫酸软骨素和硫酸角质素的免疫化学特征和超微结构定位。
DOI：
10.1007/bf00216639
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发表时间：
1988
影响因子：
3.6
通讯作者：
Caterson,B
中科院分区：
文献类型：
--
作者：
Sorrell,JM;Mahmoodian,F;Caterson,B
针对软骨素4-/硫酸皮肤素、软骨素6-硫酸、硫酸角质素上的特异性碳水化合物表位的单克隆抗体和针对透明质酸结合区的单克隆抗体用于表征从鸡胚骨髓中提取的蛋白聚糖。大约一半的蛋白聚糖在CsCl梯度上分离成高密度部分。糖胺聚糖特异性抗体识别来自所有组分的蛋白聚糖;这包括针对硫酸角质素的抗体。一些蛋白聚糖，主要是在高浮力密度部分，含有由透明质酸结合区特异性抗体识别的位点。在检测限内，所有核心蛋白都属于高分子量类别，其重量超过212 kD。针对软骨素4-/硫酸皮肤素和硫酸角质素的抗体主要结合位于细胞外间隙中的细胞外基质材料和成纤维细胞基质细胞的细胞周区域中的基质元件。识别6-硫酸软骨素的抗体与成纤维基质细胞表面的位点结合，也与细胞外基质材料结合。在粒细胞表面检测到很少或没有抗体结合。这些研究表明，硫酸软骨素和硫酸角质素链都存在于蛋白多糖提取物中。
Monoclonal antibodies directed against specific carbohydrate epitopes on chondroitin 4-/dermatan sulfate, chondroitin 6-sulfate, keratan sulfate, and a monoclonal antibody directed against the hyaluronate binding region were used to characterize proteoglycans extracted from embryonic chick bone marrow. About half of the proteoglycans separate into the high density fraction on a CsCl gradient. Glycosaminoglycan-specific antibodies recognize proteoglycans from all fractions; this includes an antibody directed against keratan sulfate. Some proteoglycans, principally in the high buoyant density fraction, contain sites recognized by the antibody specific for the hyaluronate binding region. Within limits of detection, all core proteins belong to the high-molecular-weight category, with weights in excess of 212 kD. Antibodies directed against chondroitin 4-/dermatan sulfate and against keratan sulfate primarily bind to extracellular matrix material located in the extracellular spaces and to matrix elements in the pericellular regions of fibroblastic stromal cells. The antibody that recognizes chondroitin 6-sulfate binds to sites on surfaces of fibroblastic stromal cells and also to extracellular matrix material. Little or no antibody binding is detected on surfaces of granulocytic cells. These studies indicate that chondroitin sulfate and keratan sulfate chains are both present in the proteoglycan extract.