Advances in Agrobacterium-mediated Maize Transformation.
Advances in Agrobacterium-mediated Maize Transformation.
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DOI：
10.1007/978-1-4939-7315-6_3
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发表时间：
2018
影响因子：
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通讯作者：
H. Zhong;Sivamani Elumalai;Samson Nalapalli;Lee Richbourg;A. Prairie;David Bradley;Shujie Dong;X. Su;W. Gu;Tim Strebe;Liang Shi;Q. Que
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文献类型：
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作者：
H. Zhong;Sivamani Elumalai;Samson Nalapalli;Lee Richbourg;A. Prairie;David Bradley;Shujie Dong;X. Su;W. Gu;Tim Strebe;Liang Shi;Q. Que
玉米转化的主要限制之一是使用耗时的手动提取方法分离大量未成熟胚。在这篇文章中，我们描述了一种新的大量胚提取方法，用于快速分离大量适合于基因枪和农杆菌介导的转化的胚。最佳的基因传递和组织培养条件也被描述为实现高效率的农杆菌介导的玉米转化使用磷酸甘露糖异构酶（PMI）作为选择标记。
One of the major limitations of maize transformation is the isolation of a large number of immature embryos using the time-consuming manual extraction method. In this article, we describe a novel bulk embryo extraction method for fast isolation of a large number of embryos suitable for both biolistic- andAgrobacterium-mediated transformation. Optimal gene delivery and tissue culture conditions are also described for achieving high efficiency inAgrobacterium-mediated maize transformation using phosphomannose isomerase (PMI) as a selectable marker.