CD99 expression in T-lineage ALL:: implications for flow cytometric detection of minimal residual disease
CD99 expression in T-lineage ALL:: implications for flow cytometric detection of minimal residual disease
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DOI：
10.1038/sj.leu.2403303
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发表时间：
2004-04-01
期刊：
影响因子：
11.4
通讯作者：
Gadner, H
中科院分区：
文献类型：
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作者：
Dworzak, MN;Fröschl, G;Gadner, H
CD 99在未成熟T细胞上的表达高于成熟T细胞。我们推测该标记物可用于评估T系急性淋巴细胞白血病（T-ALL）的微小残留病（MRD）。在27例T-ALL儿童的诊断性骨髓（BM）样本中，流式细胞术检测白血病淋巴母细胞上的CD 99表达，其中位数是同一样本中正常T淋巴细胞的7.7倍。在85%的病例中，白血病MFI值高于平均MFI+2 s.d.。正常人群。我们应用CD 99研究了来自15例连续T-ALL患者的39个随访样本的MRD，并将结果与已建立的MRD标志物末端脱氧核苷酸转移酶（TdT）进行了比较。在四色流式细胞术中将任一抗体与CD 7、表面CD 3和细胞质CD 3组合。我们发现，CD 99是一个有效的补充TdT定量T-ALL MRD。考虑到相当大的患者间变异性，CD 99可有利地用于9例患者，TdT可用于其他5例患者。两种方法在诊断后的12周内显示出相似的非常低的非特异性背景（非T ALL患者中有核BM细胞的中位数为0.002%）。我们的结论是，CD 99是一个高度信息化的工具MRD检测T-ALL，轴承的优势，表面表达相比TdT。
Expression of CD99 is higher on immature than on mature T cells. We postulated that this marker could be used to assess minimal residual disease (MRD) in T-lineage acute lymphoblastic leukemia (T-ALL). In diagnostic bone marrow (BM) samples from 27 children with T-ALL, expression of CD99 on leukemic lymphoblasts by flow cytometry was in median 7.7 times higher than on normal T lymphocytes from within the same sample. In 85% of cases, leukemic MFI values were higher than the mean MFI+2 s.d. of normal populations. We applied CD99 to study MRD in 39 follow-up samples from 15 consecutive T-ALL patients, and compared the results with those obtained with the well-established MRD-marker terminal deoxynucleotidyl transferase (TdT). Either antibody was combined in four-color flow cytometry with CD7, surface CD3, and cytoplasmic CD3. We found that CD99 was a valid complement to TdT in quantifying T-ALL MRD. Given a considerable interpatient variability, CD99 could be favorably used in nine patients, and TdT in other five patients. Both approaches showed a similar very low nonspecific background throughout 12 weeks from diagnosis (in median 0.002% of nucleated BM cells in patients with non-T ALL). We conclude that CD99 is a highly informative tool for MRD detection in T-ALL, bearing the advantage of surface expression in contrast to TdT.