Serum Proteins as New Biomarkers for Whole-Body Exposure to High- and Low-LET Ionizing Radiation
Serum Proteins as New Biomarkers for Whole-Body Exposure to High- and Low-LET Ionizing Radiation
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血清蛋白作为全身暴露于高 LET 和低 LET 电离辐射的新生物标志物
DOI：
10.1177/1559325820914172
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发表时间：
2020-01
期刊：
影响因子：
2.5
通讯作者：
Zhou Heng
中科院分区：
文献类型：
--
作者：
Wei Wenjun;Bai Hao;Feng Xiu;Hua Junrui;Long Kaiqin;He Jinpeng;Zhang Yanan;Ding Nan;Wang Jufang;Zhou Heng
暴露于电离辐射是对人类健康和公共安全的重大威胁。由于当前指示辐射暴露的方法存在固有的局限性，因此需要能够在早期轻松快速检测到的新的微创生物标志物，以实现最佳的医疗治疗。血清蛋白是有吸引力的生物标志物，并且已经发现了一些放射敏感性蛋白，但是响应低剂量和高线性能量转移（LET）辐射的蛋白尚未报道。在这项研究中，小鼠全身暴露于不同剂量的碳离子和 X 射线。我们进行小鼠抗体阵列检测照射后 24 小时的血清蛋白表达谱。经过条件筛选后，使用酶联免疫吸附测定进一步验证了胰岛素样生长因子-1 (IGF-1)、胰岛素样生长因子结合蛋白-1 (IGFBP-1) 和 IGFBP-3。暴露于0.05至1Gy的碳离子和0.5至4Gy的X射线后，只有IGFBP-3随碳离子和X射线剂量的增加而表现出明显的增加。此外，检测IGFBP-3以观察其随时间的变化。结果显示，碳离子和X射线照射后6至24小时，IGFBP-3的表达量出现差异。此外，接受者操作特征分析表明，血清 IGFBP-3 能够有效地以高灵敏度和特异性对暴露个体进行分类。这些结果表明，血清IGFBP-3对高和低LET电离辐射极其敏感，并且能够在早期做出反应，这可以作为辐射暴露的新型微创指标。
Exposure to ionizing radiation is a major threat to human health and public security. Since the inherent limitations of current methods for indicating radiation exposure, new minimally invasive biomarkers that can be easily and quickly detected at an early stage are needed for optimal medical treatment. Serum proteins are attractive biomarkers and some radiosensitive proteins have been found, but the proteins in response to low-dose and high-linear energy transfer (LET) radiation have not been reported. In this study, mice were whole body exposed to a variety doses of carbon ions and X-rays. We performed Mouse Antibody Array to detect serum proteins expression profiles at 24 hours postirradiation. After conditional screening, insulin-like growth factor-1 (IGF-1), insulin-like growth factor binding protein-1 (IGFBP-1), and IGFBP-3 were further validated using enzyme-linked immunosorbent assay. After exposure to 0.05 to 1 Gy of carbon ions and 0.5 to 4 Gy of X-rays, only IGFBP-3 showed obvious increase with increased doses, both carbon ions and X-rays. Further, IGFBP-3 was detected for observation of its time-dependent changes. The results showed the expression difference of IGFBP-3 presented from 6 to 24 hours post-irradiation by carbon ions and X-rays. Moreover, the receiver–operating characteristic analysis showed that serum IGFBP-3 is efficient to triage exposed individuals with high sensitivity and specificity. These results suggest that serum IGFBP-3 is extremely sensitive to high- and low-LET ionizing radiation and is able to respond at an early stage, which could serve as a novel minimally invasive indicator for radiation exposure.
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DOI：
10.5812/kowsar.1726913x.3334
发表时间：
2011-09
影响因子：
2.1
作者：
Ghollam-Reza Moshtaghi-Kashanian;F. Mirzaee;Seyyed Hossein khalilzadeh
通讯作者：
Ghollam-Reza Moshtaghi-Kashanian;F. Mirzaee;Seyyed Hossein khalilzadeh
影响因子：
3.2
作者：
Bjornsdottir, Sigridur;Oksnes, Marianne;Bensing, Sophie
通讯作者：
Bensing, Sophie
影响因子：
64.8
作者：
EVANS, HJ;BUCKTON, KE;CAROTHERS, A
通讯作者：
CAROTHERS, A
影响因子：
11.4
作者：
R. Renner
通讯作者：
R. Renner
血清 microRNA 作为电离辐射反应暴露程度估计的早期指标
影响因子：
3.4
作者：
Wei Wenjun;He Jinpeng;Wang Jufang;Ding Nan;Wang Bing;Lin Sulan;Zhang Xurui;Hua Junrui;Li He;Hu Burong
通讯作者：
Hu Burong