Thymidine labeling index, flow cytometric S-phase measurement, and DNA index in human tumors. Comparisons and correlations.
Thymidine labeling index, flow cytometric S-phase measurement, and DNA index in human tumors. Comparisons and correlations.
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人类肿瘤中的胸苷标记指数、流式细胞术 S 期测量和 DNA 指数。
DOI：
10.1093/ajcp/89.5.586
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发表时间：
1988
影响因子：
3.5
通讯作者：
Coplin,MD
中科院分区：
文献类型：
--
作者：
Meyer,JS;Coplin,MD
流式细胞术的结果进行了比较，与氚标记的胸苷标记指数（TLI）在314个肿瘤研究在一个实验室和163个肿瘤从第二个实验室，共477，包括306乳腺癌。在用碘化丙啶或DAPI染色的机械解离的新鲜组织上获得流式细胞术DNA测量。TLI与流式细胞术S期测量值（%S）的斯皮尔曼等级相关系数（r），二倍体DNA指数（DNAI）为0.38 ~ 0.55（P< 0.001），非整倍体DNA指数（DNAI）为0.59和0.67（P< 0.001）。乳腺和非乳腺肿瘤的相关性相似。非整倍体肿瘤的TLI和%S值均显著高于二倍体肿瘤（P< 0.001），但与DNA非整倍体肿瘤的TLI和%S值无关。在系列1和系列2中，乳腺癌的平均TLI分别为4.6%和3.6%;对于非整倍体肿瘤，分别为9.9%和9.3%。二倍体乳腺癌的%S分别为6.8%和7.4%，异倍体乳腺癌的%S分别为13.6%和14.5%。对于TLI低的肿瘤，%S超过TLI的程度最大，并且似乎主要来自DNA直方图中的碎片。TLI、%S和DNAI均能预测乳腺癌患者的绝对生存期和无复发生存期。
The results of flow cytometry were compared with the tritiated thymidine labeling index (TLI) in 314 tumors studied in one laboratory and 163 tumors from a second laboratory, a total of 477, including 306 breast carcinomas. Flow cytometric DNA measurements were obtained on mechanically dissociated fresh tissue stained with propidium iodide or DAPI. Spearman’s rank order correlation coefficients (r) for TLIversusflow cytometric S-phase measurement (%S) were 0.38 to 0.55 (P< 0.001) for diploid DNA index (DNAI) and 0.59 and 0.67 (P< 0.001) for aneuploid DNAI. The correlations were similar for breast and nonmammary tumors. No correlation of DNAI with TLI or %S was observed for aneuploid tumors, although the aneuploid tumors had significantly higher TLI and %S than diploid tumors (P< 0.001). Mean TLI for the breast carcinomas was 4.6% and 3.6% for diploid tumors in Series 1 and 2, respectively; for aneuploid tumors, 9.9% and 9.3%. Respective mean %S for diploid breast carcinomas was 6.8% and 7.4%; for aneuploid breast carcinomas, it was 13.6% and 14.5%. The excess of %S over TLI was greatest for tumors with low TLI and appeared to result largely from debris in DNA histograms. TLI, %S and DNAI each predicted both absolute survival and relapse-free survival of breast carcinoma patients.