Rat brain N-methyl-D-aspartate receptors expressed in Xenopus oocytes.
Rat brain N-methyl-D-aspartate receptors expressed in Xenopus oocytes.
复制标题
爪蟾卵母细胞中表达的大鼠脑 N-甲基-D-天冬氨酸受体。
DOI：
10.1126/science.2825347
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Dingledine,R
中科院分区：
文献类型：
--
作者：
Verdoorn,TA;Kleckner,NW;Dingledine,R
N-甲基-D-天冬氨酸 (NMDA) 激活一类兴奋性氨基酸受体，参与大脑中的各种塑性和病理过程。在哺乳动物神经元中对 NMDA 受体进行定量研究一直很困难，因为它通常与具有重叠药理学特异性的其他兴奋性氨基酸受体一起存在。注射了从大鼠脑原代培养物中分离出的信使 RNA 的爪蟾细胞现已用于研究 NMDA 受体。神经元 NMDA 受体的独特特性已在这种两栖动物细胞中重现，包括镁的电压依赖性阻断、NMDA 受体拮抗剂 D-2-氨基-5-磷酸戊酸的阻断以及甘氨酸的增强作用。该制剂应有助于 NMDA 受体激活调节的定量研究，并可作为纯化编码信使 RNA 的工具。
N-methyl-D-aspartate (NMDA) activates a class of excitatory amino acid receptor involved in a variety of plastic and pathological processes in the brain. Quantitative study of the NMDA receptor has been difficult in mammalian neurons, because it usually exists with other excitatory amino acid receptors of overlapping pharmacological specificities.Xenopusoocytes injected with messenger RNA isolated from primary cultures of rat brain have now been used to study NMDA receptors. The distinguishing properties of neuronal NMDA receptors have been reproduced in this amphibian cell, including voltage-dependent block by magnesium, block by the NMDA receptor antagonist D-2-amino-5-phosphonovaleric acid, and potentiation by glycine. This preparation should facilitate the quantitative study of the regulation of NMDA receptor activation and serve as a tool for purification of the encoding messenger RNA.