Temporally controlled release of multiple growth factors from a self-assembling peptide hydrogel
Temporally controlled release of multiple growth factors from a self-assembling peptide hydrogel
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DOI：
10.1088/0957-4484/27/38/385102
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发表时间：
2016-09-23
期刊：
影响因子：
3.5
通讯作者：
Nisbet, David R.
中科院分区：
文献类型：
--
作者：
Bruggeman, Kiara F.;Rodriguez, Alexandra L.;Nisbet, David R.
蛋白质生长因子已被证明具有很大的组织修复潜力，但它们固有的不稳定性和大尺寸阻止了对生物保护的神经组织的有意义的呈递。在这里，我们从自组装肽（SAP）水凝胶中创建了一个纳米纤维网络，以携带和稳定生长因子。我们显著减少了生长因子的降解，使它们的寿命延长了40倍以上。为了控制时间释放曲线，我们将多糖壳聚糖分子共价连接到生长因子上，以增加其与水凝胶纳米纤维的相互作用，并实现了4小时的延迟，证明了这种方法提供时间控制的生长因子递送的潜力。我们还描述了基于释放速率的分析，以比标准累积释放曲线允许的更详细地检查生长因子递送，并表明壳聚糖附着方法提供了更一致的释放曲线，波动减少了60%。为了证明该系统作为复杂生长因子递送平台的潜力，我们首次证明了从单个组织特异性SAP水凝胶中暂时不同地释放多种生长因子：这是再生医学的一个重要目标。
Protein growth factors have demonstrated great potential for tissue repair, but their inherent instability and large size prevents meaningful presentation to biologically protected nervous tissue. Here, we create a nanofibrous network from a self-assembling peptide (SAP) hydrogel to carry and stabilize the growth factors. We significantly reduced growth factor degradation to increase their lifespan by over 40 times. To control the temporal release profile we covalently attached polysaccharide chitosan molecules to the growth factor to increase its interactions with the hydrogel nanofibers and achieved a 4 h delay, demonstrating the potential of this method to provide temporally controlled growth factor delivery. We also describe release rate based analysis to examine the growth factor delivery in more detail than standard cumulative release profiles allow and show that the chitosan attachment method provided a more consistent release profile with a 60% reduction in fluctuations. To prove the potential of this system as a complex growth factor delivery platform we demonstrate for the first time temporally distinct release of multiple growth factors from a single tissue specific SAP hydrogel: a significant goal in regenerative medicine.