Genomic structure of the spermatid-specific Hsp70 homolog gene located in the class III region of the major histocompatibility complex of mouse and man
Genomic structure of the spermatid-specific Hsp70 homolog gene located in the class III region of the major histocompatibility complex of mouse and man
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DOI：
10.1093/oxfordjournals.jbchem.a022118
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发表时间：
1998-08-01
影响因子：
2.7
通讯作者：
Fujimoto, H
中科院分区：
文献类型：
--
作者：
Ito, Y;Ando, A;Fujimoto, H
Hsc70 t基因是在小鼠精子细胞中组成性表达的Hsp70同源基因。该基因与位于MHC III类区域的两个热诱导型Hsp 70基因Hsp 70.1和Hsp 70.3相连。人类6号染色体的同线区域包含HSPA 1B、HSPA 1A和HSPA 1L基因。在这里，我们已经从人睾丸细胞中分离出HSPA1L cDNA克隆。HSPA1L基因在起始ATG上游13 bp处含有一个内含子，Hsc70t基因也具有类似的基因组结构。Hsc70 t基因的转录起始位点位于约100 bp处。热诱导Hsp70.3基因上游600 bp，头对头连接。小鼠和人基因的序列比对显示，人HSPA1L和HSPA1A基因分别与小鼠Hsc70 t和Hsp70.3基因是直向同源的。在精子细胞特异性Hsp70基因的5'侧翼区和第一个外显子中观察到的保守序列段可能参与特异性基因表达的调控。
The Hsc70t gene is a Hsp70 homolog gene expressed constitutively in spermatids in mice. This gene is linked to two heat-inducible Hsp 70 genes, Hsp 70.1 and Hsp 70.3, located in the MHC class III region. The syntenic region of human chromosome 6 contains the HSPA1B, HSPA1A, and HSPA1L genes. Here, we have isolated a HSPA1L cDNA clone from human testicular cells. The HSPA1L gene contained an intron 13 bp upstream of the initiating ATG, A similar genomic structure was found in the Hsc70t gene. The transcription initiation site of the Hsc70t gene was located at ca. 600 bp upstream of the heat-inducible Hsp70.3 gene, linked head-to-head. Sequence alignment of the mouse and human genes revealed that the human HSPA1L and HSPA1A genes were orthologous to the mouse Hsc70t and Hsp70.3 genes, respectively. Conserved sequence stretches observed in the 5' flanking region and the first exon of the spermatid-specific Hsp70 gene may be involved in regulation of the specific gene expression.