Conversion of porcine big endothelin to endothelin by an extract from the porcine aortic endothelial cells.
Conversion of porcine big endothelin to endothelin by an extract from the porcine aortic endothelial cells.
复制标题
通过猪主动脉内皮细胞提取物将猪大内皮素转化为内皮素。
DOI：
10.1016/0006-291x(90)91751-d
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Shiro Morimoto
中科院分区：
文献类型：
--
作者：
Yasuo Matsumura;R. Ikegawa;M. Takaoka;Shiro Morimoto
用猪胸主动脉内皮细胞提取液(RIA)和反相高效液相色谱(RP-HPLC法)研究了猪大内皮素(BIG)转化为内皮素(ET)的作用。当BIG ET与提取物在酸性pH条件下与半胱氨酸蛋白酶抑制剂E-共同孵育时，孵育混合物中的免疫反应性-ET(IR-ET)量显著增加，该反应的最适pH为4.0。提取物诱导的IR-ET升高可被胃抑素-A完全抑制。这些免疫反应改变与血管收缩活动相关。将BIG ET与细胞提取物的孵育混合物用于反相高效液相色谱时，IR-ET的保留时间与人工合成的猪ET相同。我们认为胃抑素敏感的天冬氨酸蛋白酶参与了血管内皮细胞内BIG向ET的转化。
Conversion of porcine big endothelin (big ET) to endothelin (ET) by an extract from cultured porcine aortic endothelial cells was investigated using a radioimmunoassay (RIA) specific for ET and reverse-phase high performance liquid chromatography (RP-HPLC). When big ET was incubated with the extract at an acid pH in the presence of E-64, a cysteine protease inhibitor, the amount of immunoreactive-ET (IR-ET) in the incubation mixture was greatly increased and the optimum pH for this increased reaction was 4.0. The extract-induced increase in IR-ET was completely inhibited by pepstatin-A. These immunoreactive alterations correlated with those in the vasoconstrictor activity. When the incubation mixture of big ET with the cell extract was applied to the RP-HPLC, the IR-ET eluted at the same retention time as seen with synthetic porcine ET. We suggest that a pepstatin-sensitive aspartic protease is involved in the conversion of big ET to ET in vascular endothelial cells.