Cortical actin filaments in guard cells respond differently to abscisic acid in wild-type and abi1-1 mutant Arabidopsis
Cortical actin filaments in guard cells respond differently to abscisic acid in wild-type and abi1-1 mutant Arabidopsis
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DOI：
10.1007/s004250000489
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发表时间：
2001-02-01
期刊：
影响因子：
4.3
通讯作者：
Lee, Y
中科院分区：
文献类型：
--
作者：
Eun, SO;Bae, SH;Lee, Y
鸭茅保卫细胞中的皮质肌动蛋白细丝在气孔运动过程中表现出信号特有的组织[S.-O.Fun和Y.Lee(1997)Plant210：1014-1017]。为了研究肌动蛋白在信号转导中的作用，以拟南芥为材料是有利的。Heynh.，是一种优秀的模式植物，具有许多特征良好的突变体。利用免疫定位技术，我们发现海棠保卫细胞中肌动蛋白的分布与普通锦鸡儿基本相同：肌动蛋白蛋白在光照下组装成放射状细丝，并被ABA分解。此外，我们研究了ABA不敏感突变体(abil-1)中的肌动蛋白组织，以测试蛋白磷酸酶2C(PP2C)在肌动蛋白结构控制中的作用。经ABA处理后，突变体保卫细胞中既没有观察到气孔关闭，也没有观察到肌动蛋白细丝解聚。我们的结果表明，PP2C参与了ABA诱导的保卫细胞肌动蛋白的变化。
Cortical actin filaments in guard cells of Commelina communis L. show signal-specific organization during stomatal movements [S.-O. Fun and Y. Lee (1997) Plant Physiol 115: 1491-1498; S.-O. fun and Y. Lee (2000) Planta 210: 1014-1017]. To study the roles of actin in signal transduction, it is advantageous to use Arabidopsis thaliana (L.) Heynh., an excellent model plant with numerous well-characterized mutants. Using an immunolocalization technique, we found that actin deployments in guard cells of A. thaliana were basically identical to those in C. communis: actin proteins were assembled into radial filaments under illumination, and were disassembled by ABA. In addition, we examined actin organization in an ABA-insensitive mutant (abil-1) to test the involvement of protein phosphatase 2C (PP2C) in the control of actin structure. A clear difference was observed after ABA treatment, namely, neither stomatal closing nor depolymerization of actin filaments was observed in guard cells of the mutant. Our results indicate that PP2C participates in ABA-induced actin changes in guard cells.