Thrombin Ultrasensitive Detection Based on Chiral Supramolecular Assembly Signal-Amplified Strategy Induced by Thrombin-Binding Aptamer
Thrombin Ultrasensitive Detection Based on Chiral Supramolecular Assembly Signal-Amplified Strategy Induced by Thrombin-Binding Aptamer
复制标题
基于凝血酶结合适体诱导的手性超分子组装信号放大策略的凝血酶超灵敏检测
DOI：
10.1021/acs.analchem.6b04247
复制
发表时间：
2017-01-03
影响因子：
7.4
通讯作者：
Tang, Yalin
中科院分区：
文献类型：
--
作者：
Shen, Gang;Zhang, Hong;Tang, Yalin
凝血酶在止血和溶血中起关键作用。因此，开发一种高灵敏度、高选择性的凝血酶检测系统，对研究和临床诊断都具有重要意义。本文基于手性超分子组装体在生理钾背景下的信号放大策略，建立了一种检测凝血酶的新方法。该方法可以检测低至约2 pM的凝血酶浓度，并在几种常见干扰中提供高度特异性的选择性。该方法可将凝血酶与其他非特异性的类似蛋白质区分开来，并可用于稀释的真实的人血清样品中凝血酶的检测，在临床凝血酶快速检测中具有很大的应用潜力。
Thrombin plays a critical role in hemostasis and hemolysis. It is of high importance to develop a system toward thrombin detection with high sensitivity and high selectivity for both research and clinical diagnosis applications. In this paper, we developed a thrombin detection assay by taking advantage of the novel signal amplified strategy based on the chiral supramolecular assembly in physiological K background. This assay could detect thrombin as low concentration as about 2 pM and provided a highly specific selectivity among several common interferences. Furthermore, the assay can discriminate thrombin from other nonspecific analogous proteins with high selectivity and can be used to detect thrombin in diluted real human serum samples, which suggested its great potential for rapid detection of thrombin in the clinic.