SPECIFIC IMMUNOTHERAPY REDUCES THE ANTIGEN-DEPENDENT PRODUCTION OF EOSINOPHIL CHEMOTACTIC ACTIVITY FROM MONONUCLEAR-CELLS IN PATIENTS WITH ATOPIC ASTHMA
SPECIFIC IMMUNOTHERAPY REDUCES THE ANTIGEN-DEPENDENT PRODUCTION OF EOSINOPHIL CHEMOTACTIC ACTIVITY FROM MONONUCLEAR-CELLS IN PATIENTS WITH ATOPIC ASTHMA
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DOI：
10.1016/0091-6749(94)90035-3
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发表时间：
1994-08-01
影响因子：
14.2
通讯作者：
DOHI, Y
中科院分区：
文献类型：
--
作者：
NAGATA, M;SHIBASAKI, M;DOHI, Y
背景：特异性免疫治疗的疗效已得到验证。T淋巴细胞-嗜酸性粒细胞相互作用在哮喘发病机制中的作用越来越受到重视。为探讨免疫治疗对哮喘患者外周血单个核细胞（PBMC）嗜酸性粒细胞趋化活性（ECA）的影响，本研究采用粉尘螨（Dermatophagoides farinae，Df）敏感的支气管哮喘患者和健康志愿者的PBMC，分别在Df存在或不存在的情况下培养96 h。ECA在培养上清中测定通过修改Boyden的chamber method.Results：有没有显着差异，在基线水平的ECA哮喘患者与免疫治疗和那些没有免疫治疗。Df（10 ~ 10（4）ng/ml）以剂量依赖性的方式增加哮喘患者的ECA产生，Df浓度与ECA水平之间存在统计学显著相关性（r（k）= 0.34; p < 0.05）。相比之下，哮喘患者接受免疫治疗后，Df依赖性ECA的产生受到抑制，并且从紧急免疫治疗后4周开始观察到抑制作用。结论：这些结果表明免疫治疗诱导了PBMC抗原依赖性ECA产生的抑制。这可能有助于哮喘患者气道嗜酸性粒细胞浸润的减弱。
Background:The efficacy of specific immunotherapy has been verified. There is accumulating evidence focusing on the T lymphocyte-eosinophil interaction in the pathogenesis of asthma. The aim of this study was to clarify whether immunotherapy affects the production of eosinophil chemotactic activity (ECA) from peripheral blood mononuclear cells (PBMC).Methods: PBMC obtained from persons with bronchial asthma who were sensitive to Dermatophagoides farinae (Df) or from healthy volunteers were cultured for 96 hours in the presence or absence of Df. ECA in culture supernatants was assayed by means of the modified Boyden's chamber method.Results: There was no significant difference in the baseline levels of ECA between asthmatic persons treated with immunotherapy and those without immunotherapy. The addition of Df (10 to 10(4) ng/ml) enhanced the ECA production in a dose-dependent fashion in asthmatic persons without immunotherapy, and there was a statistically significant correlation between the concentrations of Df and the levels of ECA (r(k) = 0.34; p < 0.05). In contrast, Df-dependent ECA production was suppressed in asthmatic persons with immunotherapy, and the suppressive effect was observed from 4 week after rush immunotherapy.Conclusions: These results indicate that immunotherapy induces the suppression of antigen-dependent production of ECA from PBMC. This may contribute to the attenuation of eosinophil infiltration into the airways in asthma patients.