The mating-type proteins of fission yeast induce meiosis by directly activating mei3 transcription.
The mating-type proteins of fission yeast induce meiosis by directly activating mei3 transcription.
复制标题
裂殖酵母的交配型蛋白通过直接激活 mei3 转录来诱导减数分裂。
DOI：
10.1128/mcb.18.12.7317
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发表时间：
1998
影响因子：
5.3
通讯作者：
Struhl,K
中科院分区：
文献类型：
--
作者：
VanHeeckeren,WJ;Dorris,DR;Struhl,K
Cell type control of meiotic gene regulation in the budding yeastSaccharomyces cerevisiaeis mediated by a cascade of transcriptional repressors, a1-α2 and Rme1. Here, we investigate the analogous regulatory pathway in the fission yeastSchizosaccharomyces pombeby analyzing the promoter ofmei3, the single gene whose expression is sufficient to trigger meiosis. Themei3promoter does not appear to contain a negative regulatory element that represses transcription in haploid cells. Instead, correct regulation ofmei3transcription depends on a complex promoter that contains at least five positive elements upstream of the TATA sequence. These elements synergistically activatemei3transcription, thereby constituting an on-off switch for the meiosis pathway. Element C is a large region containing multiple sequences that resemble binding sites for Mc, an HMG domain protein encoded by the mating-type locus. The function of element C is extremely sensitive to spacing changes but not to linker-scanning mutations, suggesting the possibility that Mcfunctions as an architectural transcription factor. Altered-specificity experiments indicate that element D interacts with Pm, a homeodomain protein encoded by the mating-type locus. This indicates that Pmfunctions as a direct activator of the meiosis pathway, whereas the homologous mating-type protein inS. cerevisiae(α2) functions as a repressor. Thus, despite the strong similarities between the mating-type loci ofS. cerevisiaeandS. pombe, the regulatory logic that governs the tight control of the key meiosis-inducing genes in these organisms is completely different.
