Study on a Molecularly Imprinted Solid-Phase Extraction Coupled to Capillary Electrophoresis Method for the Determination of Trace Trichlorfon in Vegetables
Study on a Molecularly Imprinted Solid-Phase Extraction Coupled to Capillary Electrophoresis Method for the Determination of Trace Trichlorfon in Vegetables
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DOI：
10.1007/s12161-013-9729-y
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发表时间：
2014-07
影响因子：
2.9
通讯作者：
T. Zhao;Hui-Ju Gao;Xilong Wang;Limin Zhang;X. Qiao;Zhixiang Xu
中科院分区：
文献类型：
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作者：
T. Zhao;Hui-Ju Gao;Xilong Wang;Limin Zhang;X. Qiao;Zhixiang Xu
蔬菜中农药残留的检测是一个亟待解决的问题。建立了一种基于分子印迹聚合物的固相萃取-毛细管电泳（SPE-CE）联用测定敌百虫的新方法。优化了分子印迹固相萃取的电泳条件和影响因素。在最佳条件下，线性范围为0.1 μg/L ~ 10 mg/L。检测限（LOD）和方法定量限（MQL）分别为4.9和16.2 μg/kg。当流速为2.5 mL/min时，上样量为50 mL，富集因子为160。0.01 mg/L敌百虫标准溶液5次重复萃取的相对标准偏差（RSD）为4.5%。用该方法对黄瓜、生菜和萝卜3种敌百虫添加样品进行了提取和测定，回收率为77.6%~ 93.2%。将该方法应用于韭菜样品中敌百虫残留量的定量检测，结果与气相色谱法一致。
How to determine the pesticide residues in vegetable is an urgent problem. In this study, we reported a new method of solid-phase extraction coupled to capillary electrophoresis (SPE–CE) based on a molecularly imprinted polymer (MIP) for determination of trace trichlorfon. The electrophoretic conditions and factors which affected the molecularly imprinted solid-phase extraction were optimized. Under optimal conditions, the linear ranges of the calibration graph were 0.1 μg/L to 10 mg/L. The limit of detection (LOD) and method quantitation limit (MQL) were 4.9 and 16.2 μg/kg, respectively. With a flow rate of 2.5 mL/min for 50 mL loading, an enrichment factor of 160 was obtained. The relative standard deviation (RSD) for five replicate extractions of 0.01 mg/L trichlorfon standard solution was 4.5 %. The blank cucumber, lettuce, and radish samples spiked with trichlorfon at three levels were extracted and determined by this presented method with recoveries ranging from 77.6 to 93.2 %. Moreover, this proposed methodology was successfully applied to the quantitative detection of the trichlorfon residues in the leek samples, and the results were in good agreement with that obtained by the gas chromatography method.