Fucoidan inhibits parainfluenza virus type 2 infection to LLCMK2 cells
Fucoidan inhibits parainfluenza virus type 2 infection to LLCMK2 cells
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DOI：
10.2220/biomedres.29.331
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发表时间：
2008-12-01
影响因子：
1.2
通讯作者：
Komada, Hiroshi
中科院分区：
文献类型：
--
作者：
Taoda, Naomi;Shinji, Eri;Komada, Hiroshi
研究了岩藻依聚糖和岩藻依聚糖的基本主要成分L-岩藻糖对人副流感病毒2型（hPIV-2）在LLCMK 2细胞中生长的影响。褐藻糖胶抑制细胞融合和红细胞吸附，而L-fucosc仅部分抑制两者。在用褐藻糖胶培养的hPIV-2感染的细胞中未检测到病毒RNA。然而，L.岩藻糖不抑制病毒RNA合成。间接免疫荧光研究表明，褐藻糖胶对病毒蛋白合成有抑制作用，而L-岩藻糖对病毒蛋白合成无抑制作用。此外，使用表达绿色荧光蛋白的重组hPIV-2，发现褐藻糖胶抑制病毒进入，但不抑制L-岩藻糖。这些结果表明，岩藻依聚糖硫酸酯通过与细胞表面结合来抑制病毒吸附到细胞表面，并防止感染，表明岩藻依聚糖硫酸酯多糖形式对于岩藻依聚糖硫酸酯的抑制作用是重要的。
The effects of fucoidan and L-Fucose, a fundamental major component of Fucoidan, on the growth of human parainfluenza virus type 2 (hPIV-2) in LLCMK2 cells were investigated. Fucoidan inhibited cell fusion and hemadsorption, but L-fucosc Only partly inhibited both. Virus RNA was not detected in the hPIV-2 infected cells cultured with fucoidan. However, L.-fucose did not inhibit virus RNA synthesis. Indirect immunofluorescence study showed that virus protein synthesis was inhibited by fucoidan, but not by L-fucose. Furthermore, using a recombinant, green fluorescence protein-expressing hPIV-2, it Was found that virus entry was inhibited by fucoidan, but not by L-fucose. These results Suggested that fucoidan Inhibited Virus adsorption to the Surface of the cells by binding to the cell surface and prevented infection, indicating that the Sulfated polysaccharide form was important for the inhibition by fucoidan.