Whole-genome shotgun sequencing of mitochondria from ancient hair shafts
Whole-genome shotgun sequencing of mitochondria from ancient hair shafts
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DOI：
10.1126/science.1146971
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发表时间：
2007-09-28
期刊：
影响因子：
56.9
通讯作者：
Schuster, Stephan C.
中科院分区：
文献类型：
--
作者：
Gilbert, M. Thomas P.;Tomsho, Lynn P.;Schuster, Stephan C.
虽然合成测序技术在从骨骼中提取的DNA中的应用彻底改变了对古代DNA的研究，但它一直受到大部分污染环境DNA的困扰。毛干的遗传分析可能是一个解决方案：我们提出了10个以前未经检查的西伯利亚猛犸（猛犸象）线粒体基因组，测序覆盖率高达48倍。观察到的损伤导致的测序错误水平低于先前发表的冷冻骨样本中观察到的水平，即使其中一个样本的年龄> 50，000 C-14岁，另一个样本在室温下储存了200年。因此，该方法为博物馆藏品的分子遗传学分析奠定了基础。
Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 C-14 years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.