Investigation on Ruthenium(II) Bipyridine/AgIII Complexes Chemiluminescence System and Its Application for Sensitive Norfloxacin and Ofloxacin Detection
Investigation on Ruthenium(II) Bipyridine/AgIII Complexes Chemiluminescence System and Its Application for Sensitive Norfloxacin and Ofloxacin Detection
复制标题
联吡啶钌/Ag-III配合物化学发光系统的研究及其在诺氟沙星和氧氟沙星灵敏检测中的应用
DOI：
10.21577/0103-5053.20180223
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发表时间：
2019-04-01
影响因子：
1.4
通讯作者：
Ma, Li
中科院分区：
文献类型：
--
作者：
Liu, Jinjin;Ma, Ling;Ma, Li
研究了在酸性介质中，Ag（HIO 6）（2）]（5-）与联吡啶钌（Ru（bpy）（3）（2+））的反应机理。建立了在酸性介质中，以银-钌（II）联吡啶为显色剂，流动注射化学发光（CL）法同时测定市售药品、牛奶和人尿样中诺氟沙星（NFLX）和氧氟沙星（OFLX）的新方法。在最佳条件下，化学发光强度与药物浓度成正比，NFLX为1.6 x 10（-9）至8 x 10（-6）g mL（-1），OFLX为1.0 x 10（-9）至4 x 10（-6）g mL（-1）。NFLX和OFLX的检测限分别为1.0 x 10（-9）g mL（-1）和0.8 x 10（-9）g mL（-1）。根据化学发光光谱、紫外光谱和荧光光谱，提出了可能的反应机理。
The reaction mechanism of Ag-III complex ([Ag(HIO6)(2)](5-)) with ruthenium(II) bipyridine (Ru(bpy)(3)(2+)) in acid medium was investigated. A novel flow injection chemiluminescence (CL) analysis method was developed for the detection of norfloxacin (NFLX) and ofloxacin (OFLX) in commercial drug, milk and human urine samples based on Ag-III-ruthenium(II) bipyridine in acidic solution. Under optimal conditions, CL intensities were proportional to drug concentrations in the range of 1.6 x 10(-9) to 8 x 10(-6) g mL(-1) for NFLX and 1.0 x 10(-9) to 4 x 10(-6) g mL(-1) for OFLX. The detection limit was 1.0 x 10(-9) g mL(-1) for NFLX and 0.8 x 10(-9) g mL(-1) for OFLX. On the basis of the CL, UV and fluorescence spectra, possible reaction mechanisms were proposed.