Antioxidants promote establishment of trypanosome infections in tsetse
Antioxidants promote establishment of trypanosome infections in tsetse
复制标题
DOI：
10.1017/s0031182007002247
复制
发表时间：
2007-06-01
期刊：
影响因子：
2.4
通讯作者：
Welburn, S. C.
中科院分区：
文献类型：
--
作者：
MacLeod, E. T.;Maudlin, I.;Welburn, S. C.
通过采采蝇有效、周期性地传播锥虫对于撒哈拉以南非洲地区人类昏睡病和那加那病的维持至关重要。采采蝇的感染率通常非常低，因为大多数与苍蝇血粉一起摄入的寄生虫都会在苍蝇肠道中死亡，表现出凋亡细胞的特征。在这里，我们表明，当添加到昆虫血粉中时，一系列抗氧化剂（谷胱甘肽、半胱氨酸、N-乙酰半胱氨酸、抗坏血酸和尿酸）可以显着抑制采采蝇中布氏锥虫的细胞死亡。 L-和D-半胱氨酸都引起类似的作用，表明对锥虫死亡的抑制不依赖于蛋白质合成。目前的工作表明，抗氧化剂可以减少中肠环境，保护锥虫免受活性氧诱导的细胞死亡。
Efficient, cyclical transmission of trypanosomes through tsetse flies is central to maintenance of human sleeping sickness and nagana across sub-Saharan Africa. Infection rates in tsetse are normally very low as most parasites ingested with the fly bloodmeal die in the fly gut, displaying the characteristics of apoptotic cells. Here we show that a range of antioxidants (glutathione, cysteine, N-acetyl-cysteine, ascorbic acid and uric acid), when added to the insect bloodmeal, can dramatically inhibit cell death of Trypanosoma brucei brucei in tsetse. Both L- and D-cysteine invoked similar effects suggesting that inhibition of trypanosome death is not dependent on protein synthesis. The present work suggests that antioxidants reduce the midgut environment protecting trypanosomes from cell death induced by reactive oxygen species.