Ammonia nitrogen stress increases susceptibility to bacterial infection via blocking IL-1R-Relish axis mediated antimicrobial peptides expression in shrimp
Ammonia nitrogen stress increases susceptibility to bacterial infection via blocking IL-1R-Relish axis mediated antimicrobial peptides expression in shrimp
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DOI：
10.1016/j.aquaculture.2022.738934
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发表时间：
2022-10-19
期刊：
影响因子：
4.5
通讯作者：
Li, Chaozheng
中科院分区：
文献类型：
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作者：
Li, Haoyang;Li, Qinyao;Li, Chaozheng
氨氮的积累被认为是水产养殖系统中的一个环境胁迫因素，并已被证明增加了水生生物对感染的易感性。然而，氨氮应激导致感染或疾病暴发的易感性在很大程度上仍不清楚。在此，我们克隆并鉴定了凡纳滨对虾白介素1受体同源物(命名为LvIL-1R)，并发现了一个LvIL-1R-LvRelish轴介导的抗菌信号，该信号可诱导抗菌肽(AMPs)的表达，从而对副溶血性弧菌感染具有保护作用。此外，我们观察到在氨氮胁迫下，对虾对细菌感染更加敏感，这是由于血淋巴中氨氮浓度的升高抑制了LvIL-1R-LvRelish-AMPs抗菌轴的作用。我们的结果揭示了环境(氨氮)胁迫如何增加对虾对感染的敏感性，从而为开发有效的环境控制策略来预防和治疗水产养殖疾病提供了一些启示。
Ammonia nitrogen (ammonia-N) accumulation is pointed out as an environmental stress factor in the aquatic culture system, and has been shown to increase the susceptibility of aquatic organisms to infection. However, the mechanism by which ammonia-N stress induces a higher susceptibility to infection or disease outbreak remains largely unknown. Herein, we cloned and identified an interleukin-1 receptor homolog from the shrimp Litope-naeus vannamei (designated as LvIL-1R), and uncovered a LvIL-1R-LvRelish axis mediated antibacterial signaling that could induce the expression of antimicrobial peptides (AMPs) and thus conferred protection against Vibrio parahaemolyticus infection. Besides, we observed that shrimps became more susceptible to bacterial infection under ammonia-N stress, which attributed to that the LvIL-1R-LvRelish-AMPs antibacterial axis was restrained by the elevated ammonia-N concentration in hemolymph. Our results reveal how environmental (ammonia-N) stress increases shrimp susceptibility to infection, thereby providing some insights into the development of an effective environmental control strategy for disease prevention and treatment in aquaculture.