A protocol for generating induced T cells by reprogramming B cells in vivo
A protocol for generating induced T cells by reprogramming B cells in vivo
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通过体内重编程 B 细胞生成诱导 T 细胞的方案
DOI：
10.1016/j.cr.2018.05.001
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发表时间：
2018
影响因子：
--
通讯作者：
Wang Jinyong
中科院分区：
文献类型：
--
作者：
Weng Qitong;Hu Fangxiao;Zhang Mengyun;Dong Yong;Lv Cui;Wang Ying;Liu Xiaofei;Wang Jinyong
通过重编程获得T细胞是再生医学的主要目标之一。在这里，我们描述了一个协议，用于产生功能性T细胞从Hoxb 5表达前/前B细胞在体内。该方案包括Hoxb 5重组质粒的构建、逆转录病毒包装、pro/pre-B细胞的分离和病毒转导、细胞移植和诱导T细胞的表型分析。该过程是可重复的和简单的，提供了一种方法来产生诱导的T细胞的翻译研究。
Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cellsin vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research.