An immunoregulatory peptide from tsetse fly salivary glands of Glossina morsitans morsitans
An immunoregulatory peptide from tsetse fly salivary glands of Glossina morsitans morsitans
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DOI：
10.1016/j.biochi.2015.09.001
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发表时间：
2015-11-01
期刊：
影响因子：
3.9
通讯作者：
Rong, Mingqiang
中科院分区：
文献类型：
--
作者：
Bai, Xuewei;Yao, Huimin;Rong, Mingqiang
采采蝇 Glossina morsitans morsitans 是非洲锥虫的重要昆虫媒介，可引起人类非洲锥虫病 (HAT)。与其他食血节肢动物一样，采采蝇严重依赖唾液的药理学特性来抑制宿主的免疫反应并获取血粉。然而，关于睾蝇免疫调节剂的信息很少。从 G. morsitans morsitans（双翅目：Glossinidae）采采蝇的唾液腺中鉴定出一种名为 Gloss 2 的免疫调节肽，其含有 QKNDTAFSCHFFEIYL SNCFNKE-KYIKNYLQIM 的氨基酸序列。 Gloss 2能够抑制脂多糖（LPS）诱导的小鼠脾细胞分泌肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、白细胞介素6（IL-6）和白细胞介素10（IL-10）。此外，Gloss 2 通过阻断 JNK、Erk 和 P38 的磷酸化，显着抑制 LPS 诱导的 MAPK 信号通路激活。 Gloss 2 可能通过抑制 TNF-α、IFN-γ 和 IL-6 的分泌来抑制宿主炎症反应。考虑到 IL-10 通过增强 II 类表达 B 细胞和诱导免疫球蛋白 (Ig) 产生来促进体液免疫反应的能力，Gloss 2 可能通过抑制 IL-10 分泌来抑制宿主体液免疫反应。免疫抑制可能促进采采蝇的吸血以及非洲锥虫向宿主的传播。 (C) 2015 Elsevier B.V. 和法国 Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM)。版权所有。
Tsetse fly Glossina morsitans morsitans is an important insect vector of African trypanosomes, which cause human African trypanosomiasis (HAT). As other hematophagous arthropods, tsetse fly relies heavily on the pharmacological propriety of their saliva to suppress host's immune reactions and get blood meal. However little information is available on immune regulators from testes fly. An immunoregulatory peptide named Gloss 2 containing amino acid sequence of QKNDTAFSCHFFEIYL SNCFNKE-KYIKNYLQIM has been identified from salivary glands of the tsetse fly of G. morsitans morsitans (Diptera: Glossinidae). Gloss 2 has the ability to inhibit the secretion of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma),interleukin-6 (IL-6) and interleukin-10 (IL-10) induced by lipopolysaccharide (LPS) in mouse splenocytes. Besides, Gloss 2 significantly suppressed the LPS-induced activation of MAPK signaling pathway through blocking phosphorylations of JNK, Erk and P38. Gloss 2 probably inhibits host inflammatory responses by inhibiting secretion of TNF-alpha, IFN-gamma and IL-6. Considering IL-10's ability to promote humoral immune responses by enhancing class II expression B cells and inducing immunoglobulin (Ig) production, Gloss 2 may inhibit host humoral immune response by inhibiting IL-10 secretion. The immune-suppression may facilitate the blood feeding of tsetse fly and transmission of African trypanosomes to hosts. (C) 2015 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.