Complete Genome Sequence of Weissella hellenica 0916-4-2 and Its Comparative Genomic Analysis
Complete Genome Sequence of Weissella hellenica 0916-4-2 and Its Comparative Genomic Analysis
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DOI：
10.3389/fmicb.2019.01619
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发表时间：
2019-07-24
影响因子：
5.2
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
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作者：
Panthee, Suresh;Paudel, Atmika;Sekimizu, Kazuhisa
魏氏菌属（Weissella）属于明串珠菌科（Leuconostocaceae），是一组革兰氏阳性乳酸菌（LAB），主要存在于发酵食品中，也有部分从环境和脊椎动物（包括人类）中分离得到。目前，该属已发现23个种，16个完整的基因组和37个草图。已在各种来源中发现了希腊魏氏菌，其特征在于其益生菌和细菌素特性。尽管它的广泛的重要性，很少有人注意到这个物种的基因组特征与公众数据库中的两个物种的草案汇编到目前为止。在这份手稿中，我们确定了W。hellenica 0916-4-2，并完成了其基因组测序。比较基因组分析确定了88个核心基因，种间平均氨基酸的一致性超过65%。全基因组系统发育分析表明，3个W. hellenica菌株聚在一起，菌株0916-4-2与菌株WiKim 14接近。对次生代谢产物生物合成基因簇的硅胶分析表明，与明串珠菌科的其他成员相比，魏氏菌属的次生代谢产物产量要低得多。本研究为获得该菌的全基因组奠定了基础。hellenica 0916-4-2将促进对魏塞拉属物种的进一步比较基因组分析，包括研究其生物技术潜力和提高各种食品的营养价值。
Weissella genus from Leuconostocaceae family forms a group of Gram-positive lactic acid bacteria (LAB) that mostly reside in fermented foods and some have been isolated from the environment and vertebrates including humans. Currently there are 23 recognized species, 16 complete and 37 draft genome assemblies for this genus. Weissella hellenica has been found in various sources and is characterized by their probiotic and bacteriocinogenic properties. Despite its widespread importance, little attention has been paid to genomic characterization of this species with the availability of draft assembly of two species in the public database so far. In this manuscript, we identified W. hellenica 0916-4-2 from fermented kimchi and completed its genome sequence. Comparative genomic analysis identified 88 core genes that had interspecies mean amino acid identity of more than 65%. Whole genome phylogenetic analysis showed that three W. hellenica strains clustered together and the strain 0916-4-2 was close to strain WiKim14. In silica analysis for the secondary metabolites biosynthetic gene cluster showed that Weissella are far less producers of secondary metabolites compared to other members of Leuconostocaceae. The availability of the complete genome of W. hellenica 0916-4-2 will facilitate further comparative genomic analysis of Weissella species, including studies of its biotechnological potential and improving the nutritional value of various food products.