Streptomyces coacervatus sp nov., isolated from the intestinal tract of Armadillidium vulgare
Streptomyces coacervatus sp nov., isolated from the intestinal tract of Armadillidium vulgare
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DOI：
10.1099/ijs.0.019091-0
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发表时间：
2011-05-01
影响因子：
2.8
通讯作者：
Gonoi, Tohru
中科院分区：
文献类型：
--
作者：
Shibazaki, Azusa;Omoto, Yuta;Gonoi, Tohru
一种革兰氏染色阳性细菌，命名为AS-0823（T），它在气生菌丝体上形成螺旋孢子链，是从普通Armadillidium vulgare的肠道中分离出来的，Armadillidium vulgare是一种常见于日本房屋周围地面的小型陆生甲壳动物。16 S rRNA基因序列分析表明，该菌株属于链霉菌属，与长孢链霉菌ISP 5166（T）、Curacoi链霉菌NBRC 12761（T）和灰红链霉菌NBRC 12873（T）的16 S rRNA基因序列相似性分别为98.6%、98.4%和98.4%。菌株AS-0823（T）与链霉菌属的关系得到化学分类学数据的支持：iso-C-16：0、anteiso-C-15：0、C-16：0、iso（15：0）和anteiso-C-17：0是主要的细胞脂肪酸，LL-二氨基庚二酸是肽聚糖中的特征性二氨基酸，并且不存在分枝菌酸。DNA杂交和生理生化分析结果支持AS-0823（T）与S. longisporus JCM 4395（T）.因此，菌株AS-0823（T）代表一个新的物种，建议将其命名为Streptomyces coacervatus sp. nov.。模式菌株为AS-0823（T）（=IFM 11055（T）= DSM 41983（T）= JCM 17138（T））。
A Gram-staining-positive bacterium, designated AS-0823(T), which formed spiral spore chains on the aerial mycelium, was isolated from the intestinal tract of Armadillidium vulgare, a small terrestrial crustacean commonly found on the ground around houses in Japan. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Streptomyces and was most closely related to Streptomyces longisporus ISP 5166(T) (98.6% 16S rRNA gene sequence similarity), Streptomyces curacoi NBRC 12761(T) (98.4%) and Streptomyces griseoruber NBRC 12873(T) (98.4%). The affiliation of strain AS-0823(T) to the genus Streptomyces was supported by chemotaxonomic data: iso-C-16:0, anteiso-C-15:0, C-16:0, iso(15:0) and anteiso-C-17:0 as the major cellular fatty acids, LL-diaminopimelic acid as the characteristic diamino acid in the peptidoglycan and the absence of mycolic acids. DNA DNA hybridization and physiological and biochemical analysis supported the differentiation of strain AS-0823(T) from S. longisporus JCM 4395(T). Therefore, strain AS-0823(T) represents a novel species, for which the name Streptomyces coacervatus sp. nov. is proposed. The type strain is AS-0823(T) (=IFM 11055(T) = DSM 41983(T) = JCM 17138(T)).