The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit.
The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit.
复制标题
果蝇 110 kDa 转录因子 TFIID 亚基直接与 230 kDa 亚基的 N 末端区域相互作用。
DOI：
10.1073/pnas.90.13.5896
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发表时间：
1993
影响因子：
11.1
通讯作者：
Nakatani,Y
中科院分区：
文献类型：
--
作者：
Kokubo,T;Gong,DW;Roeder,RG;Horikoshi,M;Nakatani,Y
转录起始因子TFIID是一种多聚体蛋白复合物，通过促进启动子对各种激活剂的应答在转录调控中发挥核心作用。基于纯化蛋白的氨基酸序列，用简并寡脱氧核苷酸探针分离编码果蝇TFIID（p110）的110-kDa亚基的cDNA。整个cDNA序列包含一个开放阅读框，编码一个921个氨基酸的多肽，计算分子量为99，337 Da。通过杆状病毒载体在Sf 9细胞中表达的重组蛋白直接与TFIID（p230）的230-kDa亚基相互作用。结合先前观察到的TFIID的TATA盒结合亚基（TFIID tau或TBP）仅与TFIID亚基中的p230直接相互作用，该结果表明p110通过p230与TFIID tau形成复合物。使用各种p230突变体的结合研究表明，p110和TFIID tau都与p230的N-末端352个氨基酸部分相互作用，表明p110和TFIID tau之间通过p230相互作用进行功能性通信。
Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in transcriptional regulation by facilitating promoter responses to various activators. cDNAs encoding the 110-kDa subunit of Drosophila TFIID (p110) were isolated with a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein. The entire cDNA sequence contains an open reading frame encoding a 921-amino acid polypeptide with a calculated molecular mass of 99,337 Da. The recombinant protein expressed in Sf9 cells via a baculovirus vector interacts directly with the 230-kDa subunit of TFIID (p230). Together with the previous observation that the TATA box-binding subunit of TFIID (TFIID tau or TBP) interacts directly with only p230 among the TFIID subunits, this result suggests that p110 forms a complex with TFIID tau via p230. A binding study using various p230 mutants indicated that both p110 and TFIID tau interact with the N-terminal 352-amino acid portion of p230, suggesting a functional communication between p110 and TFIID tau via p230 interactions.