Response of Chinese hamster ovary cells to anticancer drugs under aerobic and hypoxic conditions.
Response of Chinese hamster ovary cells to anticancer drugs under aerobic and hypoxic conditions.
复制标题
在有氧和低氧条件下，中国仓鼠卵巢细胞对抗癌药的反应。
DOI：
10.1038/bjc.1981.37
复制
发表时间：
1981-02
影响因子：
8.8
通讯作者：
Guttman, P
中科院分区：
文献类型：
--
作者：
Tannock, I;Guttman, P
已知肿瘤含有耐放射治疗的缺氧细胞。实体瘤中的低增殖细胞也可能对某些药物具有耐药性，因为它们位于低药物浓度的区域，因为它们的增殖速率低（Tannock，1.968，1970; Hirst & Denekamp，1979），或者因为药物摄取或活性需要氧。相反，一些药物可能对缺氧细胞具有选择性毒性（Mohindra & Rauth，1976; Song等人，1976; Stratford等人，1980），并可能与放射或其他倾向于节省缺氧细胞的药物联合治疗。我们在下文描述了5种抗癌药物对有氧和缺氧中国仓鼠卵巢（CHO）细胞的体外作用。在我们的实验室中，CHO细胞在完全α-培养基中连续悬浮培养（Stallium等，1971），并补充了抗疟药和10%胎牛血清（FCS）。通过Mohindra & Rauth（1976）的方法，将细胞暴露于浓度为5 × 105个细胞/ml的空气或N2。在37 ° C下，在小玻璃小瓶中连续搅拌8 ml体积的培养基和细胞，并且加湿的气体混合物流过穿透每个小瓶的塞子的入口管和出口管。气体混合物为空气/500 CO2或N2/。使用5%CO2（小于10 ptS/10602）和1-2立方英尺/小时/小瓶的流速。他们是
TUMOURS ARE KNOWN to contain hypoxic cells that are resistant to treatment with radiation. Hypoxic cells in solid tumours might also be resistant to some drugs because they are situated in regions of low drug concentration, because their rate of proliferation is low (Tannock, 1.968, 1970; Hirst & Denekamp, 1979) or because oxygen is required for drug uptake or activity. Conversely, some drugs may have selective toxicity for hypoxic cells (Mohindra & Rauth, 1976; Song et al., 1976; Stratford et al., 1980) and might be used therapeutically in combination with radiation or other drugs that tend to spare hypoxic cells. We describe below the in vitro effects of 5 anti-cancer drugs on aerobic and hypoxic Chinese hamster ovary (CHO) cells. CHO cells are maintained in our laboratory in continuous suspension culturein complete a-medium (Stanners et al., 1971) supplemented with antibioticsand 10% foetal calf serum (FCS). Cells were exposed to air or N2 at a concentration of 5 x 105 cells/ml by the method of Mohindra & Rauth (1976). Medium and cells in a volume of 8 ml were stirred continuously in small glass vials at 370C, and the humidified gas mixture flowed through inlet and outlet tubes which penetrated the stoppers of each vial. Gas mixtures were air/500 CO2 or N2/. 5% CO2 (less than 10 ptS/106 02) and a flow rate of 1-2 cubic feet/h/vial was used. V'ials were