Fourier phase microscopy for investigation of biological structures and dynamics
Fourier phase microscopy for investigation of biological structures and dynamics
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DOI：
10.1364/ol.29.002503
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发表时间：
2004-11-01
期刊：
影响因子：
3.6
通讯作者：
Feld, MS
中科院分区：
文献类型：
--
作者：
Popescu, G;Deflores, LP;Feld, MS
利用傅里叶分解将低相干光象场分解成两个空间分量，它们可以相互控制地相移，从而研制出一种新型的高横向分辨率定量位相显微镜。这项技术将典型的光学显微镜转变为定量相位显微镜，具有高精度和光程灵敏度为波长/5500，可在几个小时内稳定。在上皮细胞和红细胞上获得的结果证明了该仪器在不需要样品制备的情况下定量研究与生物系统相关的结构和动力学的潜力。(C)2004年美国光学学会。
By use of the Fourier decomposition of a low-coherence optical image field into two spatial components that can be controllably shifted in phase with respect to each other, a new high-transverse-resolution quantitative-phase microscope has been developed. The technique transforms a typical optical microscope into a quantitative-phase microscope, with high accuracy and a path-length sensitivity of lambda/5500, which is stable over several hours. The results obtained on epithelial and red blood cells demonstrate the potential of this instrument for quantitative investigation of the structure and dynamics associated with biological systems without sample preparation. (C) 2004 Optical Society of America.