VASCULAR-PERMEABILITY FACTOR, AN ENDOTHELIAL-CELL MITOGEN RELATED TO PDGF
VASCULAR-PERMEABILITY FACTOR, AN ENDOTHELIAL-CELL MITOGEN RELATED TO PDGF
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DOI：
10.1126/science.2479987
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发表时间：
1989-12-08
期刊：
影响因子：
56.9
通讯作者：
CONNOLLY, DT
中科院分区：
文献类型：
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作者：
KECK, PJ;HAUSER, SD;CONNOLLY, DT
血管通透性因子（VPF）是一种40千道尔顿的二硫连接二聚体糖蛋白，在增加血管通透性、内皮细胞生长和血管生成方面具有活性。这些特性表明，肿瘤细胞表达VPF可能有助于增加与肿瘤脉管系统相关的新生血管和血管通透性。来自人U937细胞的VPF cDNA序列编码一个189个氨基酸的多肽，其结构与血小板衍生生长因子（PDGF-B） B链和其他PDGF-B相关蛋白相似。与PDGF-B的总体同一性为18%。然而，PDGF-B中的所有8个半胱氨酸在人类VPF中被发现是保守的，这表明这两种蛋白质的折叠可能是相似的。在人类VPF和PDGF-B的cooh末端一半的碱性氨基酸簇也很普遍。因此，VPF似乎与PDGF/v-sis蛋白家族有关。
Vascular permeability factor (VPF) is a 40-kilodalton disulfide-linked dimeric glycoprotein that is active in increasing blood vessel permeability, endothelial cell growth, and angiogenesis. These properties suggest that the expression of VPF by tumor cells could contribute to the increased neovascularization and vessel permeability that are associated with tumor vasculature. The cDNA sequence of VPF from human U937 cells was shown to code for a 189-amino aicd polypeptide that is similar in structure to the B chain of platelet-derived growth factor (PDGF-B) and other PDGF-B-related proteins. The overall identity with PDGF-B is 18%. However, all eight of the cysteines in PDGF-B were found to be conserved in human VPF, an indication that the folding of the two proteins is probably similar. Clusters of basic amino acids in the COOH-terminal halves of human VPF and PDGF-B are also prevalent. Thus, VPF appears to be related to the PDGF/v-sis family of proteins.