Nonhistone nuclear antigens reactive with autoantibodies. Immunofluorescence studies on distribution in synchronized cells.
Nonhistone nuclear antigens reactive with autoantibodies. Immunofluorescence studies on distribution in synchronized cells.
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DOI：
10.1083/jcb.91.3.654
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发表时间：
1981-12
期刊：
影响因子：
--
通讯作者：
Tan EM
中科院分区：
文献类型：
--
作者：
Deng JS;Takasaki Y;Tan EM
来自患有某些自身免疫性疾病的患者的血清含有针对非组蛋白核抗原的自身抗体，被用作间接免疫荧光研究中的试剂。这些核抗原的分布是在同步的人 B 淋巴细胞中确定的。使用针对Sm抗原、核核糖核蛋白复合物和SS-B抗原的自身抗体。尽管所有三种非组蛋白抗原似乎在 Go 期都显示出斑点核应变模式，但在细胞周期的其他时期也存在不同的染色模式。 SS-B 抗原在 G1/S 早期表现出明显的核仁定位。这些研究表明，某些人类疾病中出现的自身抗体可以作为核大分子的免疫组织学定位和追踪其在细胞生长和分化的不同阶段的途径的有用试剂。
Sera from patients with certain autoimmune diseases tht contained autoantibodies to nonhistone nuclear antigens were used as reagents in an indirect immunofluorescent study. The distribution of these nuclear antigens was determined in synchronized human B lymphoid cells. Autoantibodies to Sm antigen, nuclear ribonucleoprotein complex and SS- B antigen were used. Although all three nonhistone antigens appeared to show speckled nuclear straining patterns in the Go phase, different patterns of staining were present at other periods of the cell cycle. The SS-B antigen showed a distinctly nucleolar localization during the G1/early S phase. These studies demonstrate that autoantibodies occurring in certain human diseases can be useful reagents for the immunohistological localization of nuclear macromolecules and for tracing their pathways during different phases of cell growth and differentiation.