Nasal biomarker profiles in acute and chronic rhinosinusitis
Nasal biomarker profiles in acute and chronic rhinosinusitis
复制标题
DOI：
10.1111/j.1365-2222.2005.02316.x
复制
发表时间：
2005-09-01
影响因子：
6.1
通讯作者：
Bürner, H
中科院分区：
文献类型：
--
作者：
Riechelmann, H;Deutschle, T;Bürner, H
研究背景鼻窦炎的临床表现包括急性鼻窦炎、慢性鼻窦炎伴鼻息肉和慢性鼻窦炎不伴鼻息肉3种类型。12例无息肉的CRS患者，13例有息肉的CRS患者和6例健康对照。结果与对照组相比，鼻窦炎患者的各项生物标志物均显著升高（P < 0.001），而与临床表现无关。主成分分析允许提取三个因素解释83%的数据方差。全身炎症活动主要由第一因子反映。第二个因素区分急性和CRS。该因子与IL-12相关，IL-12参与抗原呈递细胞的病原体相关免疫激活。与IL-4、IL-10、IL-13呈正相关，在感染消退中起重要作用。第三个因素区分CRS息肉和CRS无息肉（P=0.001）。其代表IL-5和鼻IgE（nIgE），而嗜酸性粒细胞阳离子蛋白和类胰蛋白酶对CRS伴息肉无特异性。少数生物标志物与特定疾病过程的简单相关性具有高估可能非特异性效应的风险。为了评估生物标志物谱，更复杂的分析工具可能更适合于描述粘膜疾病的潜在机制。使用主成分分析，发现高nIgE和IL-5水平是CRS伴鼻息肉的特异性。
Background Clinical manifestations of rhinosinusitis include acute rhinosinusitis, chronic rhinosinusitis (CRS) with nasal polyps and CRS without polyps.Objective Possible mechanisms defining these three forms of rhinosinusitis should be investigated assessing biomarker profiles in nasal secretions.Methods Fifteen cytokines, three cellular activation markers and total IgE were determined in nasal secretions of seven patients with acute rhinosinusitis, 12 patients with CRS without polyps, 13 patients with CRS with polyps and six healthy controls. Principal component analysis was used to extract relevant factors.Results Irrespective of the clinical manifestation, all biomarkers assessed were increased in patients with rhinosinusitis when compared with controls (P < 0.001). Principal component analysis allowed the extraction of three factors explaining 83% of data variance. The general inflammatory activation was mainly reflected by the first factor. The second factor differentiated acute from CRS. This factor correlated with IL-12, which is involved in pathogen-related immune activation by antigen-presenting cells. It was also positively correlated with IL-4, IL-10 and IL-13, which play an important role in the resolution of infections. The third factor differentiated CRS with polyps from CRS without polyps (P=0.001). It represented IL-5 and nasal IgE (nIgE), whereas eosinophil cationic protein and tryptase were not specific for CRS with polyps.Conclusion In mucosal infection, numerous inflammatory mediators are activated. Simple correlations of few biomarkers with a specific disease process bear the risk of overestimating a possibly unspecific effect. To assess biomarker profiles, more complex analytic tools may be more appropriate to delineate mechanisms underlying mucosal disease. Using principal component analysis, it was found that high nIgE and IL-5 levels are specific for CRS with nasal polyps.