RAT INTESTINAL GLYCOLIPIDS .3. FATTY-ACIDS AND LONG-CHAIN BASES OF GLYCOLIPIDS FROM VILLUS AND CRYPT CELLS
RAT INTESTINAL GLYCOLIPIDS .3. FATTY-ACIDS AND LONG-CHAIN BASES OF GLYCOLIPIDS FROM VILLUS AND CRYPT CELLS
复制标题
DOI：
10.1016/0005-2760(77)90043-1
复制
发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
GLICKMAN, RM
中科院分区：
文献类型：
--
作者：
BOUHOURS, JF;GLICKMAN, RM
以往的研究表明，大鼠小肠糖脂分化绒毛细胞和未成熟的隐窝细胞之间的显着差异。绒毛细胞含有比例更高的葡萄糖神经酰胺和血苷，而隐窝细胞缺乏血苷，但含有比例更高的三己糖神经酰胺。为了进一步阐明绒毛和隐窝细胞糖脂之间可能存在的差异，对大鼠肠糖脂的鞘氨醇和脂肪酸进行了研究。从大鼠小肠分离绒毛和隐窝细胞，并纯化糖脂。从这些细胞中提取的3种主要糖脂（葡萄糖神经酰胺，三己糖神经酰胺，血苷）的脂肪酸和长链碱进行了表征。无论是绒毛还是隐窝细胞，植物鞘氨醇占所有糖脂中总长链碱基的63-73%。羟基脂肪酸占葡萄糖神经酰胺和血苷中总脂肪酸的70%，但在三己糖神经酰胺中仅占30%。此外，trihexosylceramide含有较大比例的脂肪酸与20个碳原子比葡萄糖神经酰胺和从绒毛细胞分离的hematoside。这些脂肪酸更集中在隐窝细胞比绒毛细胞糖脂。分别在绒毛和隐窝细胞中丰富的血红素和三己糖神经酰胺可能来源于不同的乳糖神经酰胺前体，并进一步强调绒毛和隐窝细胞糖脂合成的差异。
Previous studies have demonstrated a striking difference in rat intestinal glycolipids between differentiated villus cells and immature crypt cells. Villus cells contained proportionally greater amounts of glucosylceramide and hematoside while crypt cells were deficient in hematoside, but contained proportionally greater amounts of trihexosylceramide. In order to further elucidate possible differences between villus and crypt cell glycolipids, a study of the sphingosine and fatty acids of rat intestinal glycolipids was conducted. Villus and crypt cells were separated from rat intestine and the glycolipids purified. Fatty acids and long chain bases of the 3 major glycolipids (glucosylceramide, trihexosylceramide, hematoside) extracted from these cells were characterized. Phytosphingosine accounted for 63-73% of the total long chain bases in all glycolipids whether from villus or crypt cells. Hydroxy fatty acids represented 70% of total fatty acids in the glucosylceramide and in the hematoside but accounted for only 30% in the trihexosylceramide. In addition, trihexosylceramide contained a larger percentage of fatty acids with 20-carbon atoms than glucosylceramide and hematoside isolated from villus cells. These fatty acids were more concentrated in crypt cells than in villus cells glycolipids. Hematoside and trihexosylceramide, respectively, abundant in villus and in crypt cells, may be derived from a different lactosylceramide precursor and further underscore differences in villus and crypt cell glycolipid synthesis.