HUMAN DERMAL MAST-CELLS CONTAIN AND RELEASE TUMOR-NECROSIS-FACTOR-ALPHA, WHICH INDUCES ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1
HUMAN DERMAL MAST-CELLS CONTAIN AND RELEASE TUMOR-NECROSIS-FACTOR-ALPHA, WHICH INDUCES ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1
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DOI：
10.1073/pnas.88.10.4220
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发表时间：
1991-05-01
影响因子：
11.1
通讯作者：
MURPHY, GF
中科院分区：
文献类型：
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作者：
WALSH, LJ;TRINCHIERI, G;MURPHY, GF
肿瘤坏死因子-α（TNF-α）是一种促炎细胞因子，通过诱导粘附分子的表达介导内皮白细胞相互作用。 在这份报告中，我们证明，人真皮肥大细胞含有大量的免疫反应性和生物活性的TNF-α颗粒内，它可以迅速释放到细胞外空间脱粒。 在正常人真皮细胞中，肥大细胞是表达TNF-α蛋白和TNF-α mRNA的主要细胞类型。 此外，内皮白细胞粘附分子1表达的诱导是肥大细胞源性TNF-α释放的直接结果。 这些发现确立了人肥大细胞作为真皮微血管系统“守门人”的作用，并表明肥大细胞产物而不是血管活性胺以促炎方式影响内皮。
Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine that mediates endothelial leukocyte interactions by inducing expression of adhesion molecules. In this report, we demonstrate that human dermal mast cells contain sizeable stores of immunoreactive and biologically active TNF-alpha within granules, which can be released rapidly into the extracellular space upon degranulation. Among normal human dermal cells, mast cells are the predominant cell type that expresses both TNF-alpha protein and TNF-alpha mRNA. Moreover, induction of endothelial leukocyte adhesion molecule 1 expression is a direct consequence of release of mast cell-derived TNF-alpha. These findings establish a role for human mast cells as "gatekeepers" of the dermal microvasculature and indicate that mast cell products other than vasoactive amines influence endothelium in a proinflammatory fashion.