Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale.
Using high-speed videography for objective and reproducible pain measurement on a mouse pain scale.
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DOI：
10.1016/j.xpro.2021.100322
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Abdus-Saboor I
中科院分区：
文献类型：
--
作者：
Burdge J;Fried NT;Abdus-Saboor I
小鼠模型对于研究疼痛神经生物学和测试疼痛疗法是必不可少的。对只测量反射的存在、缺失或频率的分析的依赖限制了临床前疼痛研究的可靠性。我们的高速摄像协议克服了这一点，通过将后爪刺激诱导的离散亚秒级运动行为特征投影到“老鼠疼痛刻度”上。这通过量化刺激诱导的后爪反射的质量，为小鼠提供了更客观和更稳健的疼痛测量方法。有关本议定书的使用和执行的完整细节，请参阅。爪子撤退反射的高速成像增强了小鼠疼痛评估的分辨率四个亚秒级特征区分由伤害性刺激和无害刺激引起的撤退主成分分析将行为特征投影到单轴疼痛等级协议只需要500-1000帧/秒的相机和标准的临床前疼痛相关刺激小鼠模型对于研究疼痛神经生物学和测试疼痛疗法至关重要。对只测量反射的存在、缺失或频率的分析的依赖限制了临床前疼痛研究的可靠性。我们的高速摄像协议克服了这一点，通过将后爪刺激诱导的离散亚秒级运动行为特征投影到“老鼠疼痛刻度”上。这通过量化刺激诱导的后爪反射的质量，为小鼠提供了更客观和更稳健的疼痛测量方法。
Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by projecting the discrete sub-second kinematic behavioral features induced by hind paw stimulation onto a “mouse pain scale.” This provides a more objective and robust pain measurement in mice by quantifying the quality of the stimulus-induced hind paw reflex. For complete details on the use and execution of this protocol, please refer to. High-speed imaging of paw withdrawal reflex enhances resolution of mouse pain assessment Four sub-second features distinguish withdrawals induced by noxious vs innocuous stimuli Principal component analysis projects behavioral features onto a single axis pain scale Protocol only requires 500–1,000 fps camera and standard preclinical pain-related stimuli Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by projecting the discrete sub-second kinematic behavioral features induced by hind paw stimulation onto a “mouse pain scale.” This provides a more objective and robust pain measurement in mice by quantifying the quality of the stimulus-induced hind paw reflex.
影响因子：
7.7
作者：
Jones, Jessica M.;Foster, William;Abdus-Saboor, Ishmail
通讯作者：
Abdus-Saboor, Ishmail
影响因子：
8.8
作者：
Abdus-Saboor, Ishmail;Fried, Nathan T.;Luo, Wenqin
通讯作者：
Luo, Wenqin