PHYSICOCHEMICAL BASIS OF CHOLESTEROL GALLSTONE FORMATION IN MAN
PHYSICOCHEMICAL BASIS OF CHOLESTEROL GALLSTONE FORMATION IN MAN
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DOI：
10.1172/jci105794
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发表时间：
1968-01-01
影响因子：
15.9
通讯作者：
SMALL, DM
中科院分区：
文献类型：
--
作者：
ADMIRAND, WH;SMALL, DM
测定了66份胆固醇结石患者胆囊胆汁和25份正常胆囊胆汁中胆盐、卵磷脂和胆固醇的浓度。当这3种成分同时绘制在三角坐标上时，实现了正常和“异常”胆汁的完全分离。这种分离是胆固醇结石患者胆汁中胆固醇含量相对于胆盐和卵磷脂含量增加的结果。构建了体外模型系统（在三角坐标上），该系统可以预测胆固醇在含有不同比例胆盐和卵磷脂的溶液中的最大溶解量。当胆汁数据与来自模型系统的胆固醇的溶解度进行比较时，发现正常胆汁中胆固醇不饱和，而胆固醇结石患者的胆汁饱和，并且在某些情况下含有微晶形式的不溶性胆固醇。胆汁的物理状态（i.例如，不溶性胆固醇的存在或不存在）由胆汁盐、卵磷脂和胆固醇的相对浓度决定，并且其它胆汁成分似乎不显著影响胆固醇在胆汁中的溶解度。
The concentrations of bile salt, lecithin, and cholesterol were determined on each of 66 samples of gall bladder bile from patients with cholesterol gallstones and 25 samples of normal gall bladder bile. When these 3 constituents were plotted simultaneously on triangular coordinates, a complete separation of the normal and "abnormal" bile was achieved. This separation was the result of an increase in the quantity of cholesterol relative to the amounts of bile salts and lecithin contained in the bile from patients with cholesterol gallstones. An in vitro model system was constructed (on triangular coordinates) that allows prediction of the maximum amount of cholesterol that can be solubilized in solutions containing varying proportions of bile salt and lecithin. When the bile data were compared with the solubility of cholesterol derived from the model system, normal biles were found to be less than saturated with cholesterol, whereas biles from patients with cholesterol gallstones were saturated and in some cases contained insoluble cholesterol in the form of microcrystals. The physical state of bile (i. e., the presence or absence of insoluble cholesterol) is determined by the relative concentrations of bile salt, lecithin, and cholesterol, and the other biliary constituents do not appear to significantly affect the solubility of cholesterol in bile.