Telemetric monitoring of 24 h intraocular pressure in conscious and freely moving C57BL/6J and CBA/CaJ mice
Telemetric monitoring of 24 h intraocular pressure in conscious and freely moving C57BL/6J and CBA/CaJ mice
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发表时间：
2008-04
期刊：
影响因子：
2.2
通讯作者：
Ruixia Li;John H. K. Liu
中科院分区：
文献类型：
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作者：
Ruixia Li;John H. K. Liu
目的 利用遥测技术研究清醒且自由活动的小鼠 24 小时眼压 (IOP) 的变化模式。方法 将成年 C57BL/6J 和 CBA/CaJ 小鼠置于标准的 12 小时光照和 12 小时黑暗循环中。将遥测压力发射器皮下植入每只明暗夹带小鼠的上背部，并将压力导管尖端插入玻璃体室。广播的 IOP 数据以 120 Hz 接收。每 5 分钟记录一次 2 分钟 IOP 平均值，持续 4-13 天，以生成每个小鼠品系的 24 小时 IOP 模式。 C57BL/6J 品系的 IOP 模式也在急性持续黑暗条件下 24 小时进行测定。结果 C57BL/6J 和 CBA/CaJ 小鼠品系的 24 小时 IOP 具有不同的模式。在标准明暗条件下，两种菌株的眼压在黑暗期均高于光照期。 CBA/CaJ品系从亮期到暗期的IOP升高（1.6±1.7mmHg，平均值±标准差（SD），n=21）显着小于C57BL/6J品系（3.4±2.5mmHg，n=20）。 C57BL/6J 品系的 24 小时 IOP 模式在急性恒定黑暗条件下持续存在 (n=8)。结论 两种小鼠品系的24 h IOP出现明显的变化模式。尽管两种品系在黑暗期的平均 IOP 均显着高于光照期，但明暗期 IOP 升高的幅度不同。在没有光的情况下，24 小时 IOP 变化模式可以由内源性驱动。
Purpose To study change patterns of 24 h intraocular pressure (IOP) in conscious and freely moving mice using telemetry. Methods Adult C57BL/6J and CBA/CaJ mice were entrained to a standard 12 h light and 12 h dark cycle. A telemetric pressure transmitter was implanted subcutaneously on the upper back of each light-dark entrained mouse, and the pressure catheter tip was inserted into the vitreous chamber. Broadcasted IOP data were received at 120 Hz. Means of 2 min IOP were recorded every 5 min for 4–13 days to generate the 24 h IOP pattern in each mouse strain. The pattern of IOP in the C57BL/6J strain was also determined under an acute constant dark condition for 24 h. Results There were distinct patterns of 24 h IOP in the C57BL/6J and CBA/CaJ mouse strains. Under the standard light-dark condition, IOP was higher during the dark period than the light period in both strains. Elevation in IOP from the light period to the dark period was significantly smaller in the CBA/CaJ strain (1.6±1.7 mmHg, mean± standard deviation (SD), n=21) than in the C57BL/6J strain (3.4±2.5 mmHg, n=20). The 24 h IOP pattern in the C57BL/6J strain persisted under an acute constant dark condition (n=8). Conclusions Distinct change patterns of 24 h IOP appeared in these two mouse strains. Although mean IOP during the dark period was significantly higher than the light period in both strains, the magnitudes of light-dark IOP elevation differed. The 24 h IOP change pattern can be driven endogenously in the absence of light.