Action potentials induced in guinea pig arterial smooth muscle by tetraethylammonium.
Action potentials induced in guinea pig arterial smooth muscle by tetraethylammonium.
复制标题
四乙铵诱导豚鼠动脉平滑肌的动作电位。
DOI：
10.1152/ajpcell.1979.237.1.c75
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
N. Sperelakis
中科院分区：
文献类型：
--
作者：
D. Harder;N. Sperelakis
方法甲氧基氟醚麻醉豚鼠，取肠系膜上动脉2 ~ 3cm段（直径500 ~ 600um）。这些片段被切开，形成一个薄片，允许微电极从容器的内表面渗透。在解剖显微镜下可见的结缔组织纵链之间进行微电极穿透。将动脉条置于37℃的非再循环器官浴中（体积10ml），用生理盐溶液持续灌注，盐溶液含有（mM）： 141 Na ', 4.7 K ', 1.8 Ca2 ', 0.76 Mg ' +, 124 Cl-, 1.7 HZPOQ, 25 HCO $和11葡萄糖。所有解决方案
METHODSGuinea pigs were anesthetized with methoxyflurane, and a 2-to 3-cm segment of superior mesenteric artery (500-600, um in diameter) was removed. The segments were slit open to form a sheet to allow microelectrode, penetration from the internal surface of the vessel. Microelectrode penetrations were made between the longitudinal strands of connective tissue that were visible under a dissecting microscope. The arterial strip was placed in a nonrecirculating organ bath (vol 10 ml) at 37OC for continual suffusion with a physiologic salt solution containing (mM): 141 Na’, 4.7 K’, 1.8 Ca2’, 0.76 Mg”+, 124 Cl-, 1.7 HZPOQ, 25 HCO $ and 11 glucose. All solutions